Human Population Genetic Studies of Five Hypervariable DNA Loci

Overview

Journal Am J Hum Genet

Publisher Cell Press

Specialty Genetics

Date 1989 Feb 1

PMID 2563192

Citations 45

Authors

I Balazs

M Baird

M Clyne

E Meade

Affiliations

Soon will be listed here.

Abstract

Population genetic studies were performed using DNA probes that recognize five hypervariable loci (D2S44, D14S1, D14S13, D17S79, and DXYS14) in the human genome. DNA from approximately 900 unrelated individuals, subdivided into three ethnic groups (American blacks, Caucasians, and Hispanics) were digested with PstI and were successively hybridized to each DNA probe. The number of distinct DNA fragments identified for each of these regions varies from 30 to more than 80. An allele frequency distribution was determined for each locus and each ethnic group. The results show significant differences, between ethnic groups, in the pattern of distribution as well as in the relative frequency of the most common alleles of D2S44, D14S1, and D14S13 but only small differences in others (i.e., D17S79 and DXYS14). The results presented show that the analysis of these loci can have useful applications in population genetics as well as in identity tests.

Citing Articles

DNA fingerprinting in anthropological genetics: past, present, future.

Crawford M, Beaty K Investig Genet. 2013; 4(1):23.

PMID: 24245746 PMC: 3831593. DOI: 10.1186/2041-2223-4-23.

BamHI polymorphism of locus D2S44 in a West German population as revealed by VNTR probe YNH24.

Henke L, Cleef S, Zakrzewska M, Henke J Int J Legal Med. 1990; 104(1):33-8.

PMID: 11453090 DOI: 10.1007/BF01816481.

Quantitative evaluation of post-bone marrow transplant engraftment status using fluorescent-labeled variable number of tandem repeats.

Luhm R, Bellissimo D, Uzgiris A, Drobyski W, Hessner M Mol Diagn. 2000; 5(2):129-38.

PMID: 11066014 DOI: 10.1007/BF03262031.

Hardy-Weinberg testing for continuous data.

McIntyre L, Weir B Genetics. 1997; 147(4):1965-75.

PMID: 9409851 PMC: 1208361. DOI: 10.1093/genetics/147.4.1965.

Improved single-strand DNA sizing accuracy in capillary electrophoresis.

Rosenblum B, Oaks F, Menchen S, Johnson B Nucleic Acids Res. 1997; 25(19):3925-9.

PMID: 9380518 PMC: 146964. DOI: 10.1093/nar/25.19.3925.

References

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Bell G, Selby M, Rutter W . The highly polymorphic region near the human insulin gene is composed of simple tandemly repeating sequences. Nature. 1982; 295(5844):31-5. DOI: 10.1038/295031a0. View

Capon D, Chen E, Levinson A, Seeburg P, Goeddel D . Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue. Nature. 1983; 302(5903):33-7. DOI: 10.1038/302033a0. View

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

Ginsburg D, Antin J, Smith B, Orkin S, Rappeport J . Origin of cell populations after bone marrow transplantation. Analysis using DNA sequence polymorphisms. J Clin Invest. 1985; 75(2):596-603. PMC: 423535. DOI: 10.1172/JCI111736. View

Cooke H, Brown W, Rappold G . Hypervariable telomeric sequences from the human sex chromosomes are pseudoautosomal. Nature. 1985; 317(6039):687-92. DOI: 10.1038/317687a0. View

Kanter E, Baird M, Shaler R, Balazs I . Analysis of restriction fragment length polymorphisms in deoxyribonucleic acid (DNA) recovered from dried bloodstains. J Forensic Sci. 1986; 31(2):403-8. View

Giusti A, Baird M, Pasquale S, Balazs I, Glassberg J . Application of deoxyribonucleic acid (DNA) polymorphisms to the analysis of DNA recovered from sperm. J Forensic Sci. 1986; 31(2):409-17. View

Ito H, Yasuda N, Matsumoto H . The probability of parentage exclusion based on restriction fragment length polymorphisms. Jinrui Idengaku Zasshi. 1985; 30(4):261-9. DOI: 10.1007/BF01907963. View

10.

Jarman A, Nicholls R, Weatherall D, Clegg J, Higgs D . Molecular characterisation of a hypervariable region downstream of the human alpha-globin gene cluster. EMBO J. 1986; 5(8):1857-63. PMC: 1167051. DOI: 10.1002/j.1460-2075.1986.tb04437.x. View

11.

Baird M, Balazs I, Giusti A, Miyazaki L, NICHOLAS L, Wexler K . Allele frequency distribution of two highly polymorphic DNA sequences in three ethnic groups and its application to the determination of paternity. Am J Hum Genet. 1986; 39(4):489-501. PMC: 1683978. View

12.

Nakamura Y, Leppert M, OConnell P, Wolff R, Holm T, Culver M . Variable number of tandem repeat (VNTR) markers for human gene mapping. Science. 1987; 235(4796):1616-22. DOI: 10.1126/science.3029872. View

13.

Wainscoat J, Pilkington S, Peto T, Bell J, Higgs D . Allele-specific DNA identity patterns. Hum Genet. 1987; 75(4):384-7. DOI: 10.1007/BF00284114. View

14.

Cooke H, Smith B . Variability at the telomeres of the human X/Y pseudoautosomal region. Cold Spring Harb Symp Quant Biol. 1986; 51 Pt 1:213-9. DOI: 10.1101/sqb.1986.051.01.026. View

15.

Jeffreys A, Royle N, Wilson V, Wong Z . Spontaneous mutation rates to new length alleles at tandem-repetitive hypervariable loci in human DNA. Nature. 1988; 332(6161):278-81. DOI: 10.1038/332278a0. View

16.

Fisher R . Standard calculations for evaluating a blood-group system. Heredity (Edinb). 1951; 5(1):95-102. DOI: 10.1038/hdy.1951.5. View