Transient Gene Expression in Aleurone Protoplasts Isolated from Developing Caryopses of Barley and Wheat

Overview

Journal Plant Mol Biol

Date 1989 Jul 1

PMID 2562757

Citations 15

Authors

B Lee

K Murdoch

J Topping

M Kreis

M G Jones

Affiliations

Soon will be listed here.

Abstract

Methods have been developed for the isolation of aleurone protoplasts from developing caryopses of Hordeum vulgare and Triticum aestivum in order to study transient expression of introduced genes. Chimaeric gene constructs were introduced into aleurone protoplasts by polyethylene glycol (PEG). Transient expression directed by the 35S promoter from cauliflower mosaic virus (CaMV) of the reporter gene encoding chloramphenicol acetyl transferase (CAT) was detected in aleurone protoplasts from developing barley and wheat grains. Using a similar construct, CAT activity increased when the alcohol dehydrogenase intron 1 fragment from maize was ligated between the 35S promoter and the CAT coding region. The demonstration of transient expression in protoplasts from developing aleurone layers indicates that they may be useful for investigating tissue and developmental control of genes coding for cereal seed proteins.

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References

Colot V, Robert L, Kavanagh T, Bevan M, Thompson R . Localization of sequences in wheat endosperm protein genes which confer tissue-specific expression in tobacco. EMBO J. 1987; 6(12):3559-64. PMC: 553821. DOI: 10.1002/j.1460-2075.1987.tb02685.x. View

Schernthaner J, Matzke M, Matzke A . Endosperm-specific activity of a zein gene promoter in transgenic tobacco plants. EMBO J. 1988; 7(5):1249-55. PMC: 458370. DOI: 10.1002/j.1460-2075.1988.tb02938.x. View

Junker B, Zimny J, Luhrs R, Lorz H . Transient expression of chimaeric genes in dividing and non-dividing cereal protoplasts after PEG-induced DNA uptake. Plant Cell Rep. 2013; 6(5):329-32. DOI: 10.1007/BF00269552. View

Walker J, Howard E, Dennis E, Peacock W . DNA sequences required for anaerobic expression of the maize alcohol dehydrogenase 1 gene. Proc Natl Acad Sci U S A. 1987; 84(19):6624-8. PMC: 299135. DOI: 10.1073/pnas.84.19.6624. View

Ou-Lee T, Turgeon R, Wu R . Expression of a foreign gene linked to either a plant-virus or a Drosophila promoter, after electroporation of protoplasts of rice, wheat, and sorghum. Proc Natl Acad Sci U S A. 1986; 83(18):6815-9. PMC: 386600. DOI: 10.1073/pnas.83.18.6815. View

Jacobsen J, Zwar J, Chandler P . Gibberellic-acid-responsive protoplasts from mature aleurone of Himalaya barley. Planta. 2013; 163(3):430-8. DOI: 10.1007/BF00395154. View

Negrutiu I, Shillito R, Potrykus I, Biasini G, Sala F . Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts. Plant Mol Biol. 2013; 8(5):363-73. DOI: 10.1007/BF00015814. View

Norman H, Black M, Chapman J . The induction of sensitivity to gibberellin in aleurone tissue of developing wheat grains : III. Sensitisation of isolated protoplasts. Planta. 2013; 158(3):264-71. DOI: 10.1007/BF01075263. View

Marris C, Gallois P, Copley J, Kreis M . The 5' flanking region of a barley B hordein gene controls tissue and developmental specific CAT expression in tobacco plants. Plant Mol Biol. 2013; 10(4):359-66. DOI: 10.1007/BF00029886. View

10.

Hauptmann R, Ozias-Akins P, Vasil V, Tabaeizadeh Z, Rogers S, Horsch R . Transient expression of electroporated DNA in monocotyledonous and dicotyledonous species. Plant Cell Rep. 2013; 6(4):265-70. DOI: 10.1007/BF00271995. View

11.

Lindsey K, Jones M . Transient gene expression in electroporated protoplasts and intact cells of sugar beet. Plant Mol Biol. 2013; 10(1):43-52. DOI: 10.1007/BF00014185. View

12.

Hooley R . Protoplasts isolated from aleurone layers of wild oat (Avena fatua L.) exhibit the classic response to gibberellic acid. Planta. 2013; 154(1):29-40. DOI: 10.1007/BF00385493. View

13.

Callis J, Fromm M, Walbot V . Introns increase gene expression in cultured maize cells. Genes Dev. 1987; 1(10):1183-200. DOI: 10.1101/gad.1.10.1183. View

14.

Gorman C, Moffat L, Howard B . Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982; 2(9):1044-51. PMC: 369897. DOI: 10.1128/mcb.2.9.1044-1051.1982. View

15.

Fromm M, Taylor L, Walbot V . Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc Natl Acad Sci U S A. 1985; 82(17):5824-8. PMC: 390645. DOI: 10.1073/pnas.82.17.5824. View

16.

Klein T, Fromm M, Weissinger A, Tomes D, Schaaf S, Sletten M . Transfer of foreign genes into intact maize cells with high-velocity microprojectiles. Proc Natl Acad Sci U S A. 1988; 85(12):4305-9. PMC: 280417. DOI: 10.1073/pnas.85.12.4305. View