Apoptosis and Expression of Argyrophilic Nucleolus Organizer Regions in Epithelial Neoplasms of the Larynx

Overview

Journal Braz J Otorhinolaryngol

Specialty Otorhinolaryngology

Date 2015 Jan 25

PMID 25617064

Citations 2

Authors

Christiana Vargas Ribeiro

Anilton Cesar Vasconcelos

Jose de Souza Andrade Filho

Affiliations

Soon will be listed here.

Abstract

Introduction: Occurrence of apoptosis and expression of proliferative markers are powerful tools to establish a prognosis in the follow-up of cancer.

Objective: To evaluate the growth fraction in papillomas and laryngeal squamous cell carcinomas with three degrees of differentiation through apoptosis and the expression of nucleolus organizer regions.

Methods: Retrospective study from which paraffin material was submitted to microtomy and hematoxylin-eosin and silver staining. Stained slides were used to quantify the apoptotic index and the number of nucleolus organizer regions by morphometry.

Results: Apoptosis was significantly more frequent in well differentiated carcinomas and in papillomas, and a higher growth fraction of expressed nucleolus organizer regions and cells that expressed a greater than average number of nucleolus organizer regions were more frequently noted in undifferentiated carcinomas.

Conclusions: Thus, it was possible to verify that a high apoptotic index was associated with a lower chance of tumor differentiation in carcinomas, while a greater number of total nucleolus organizer regions, cells expressing nucleolus organizer regions above average and a higher growth fraction were associated with greater likelihood of abnormal cell proliferation and increased tumor differentiation.

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