Chitosan Hydrogels for Chondroitin Sulphate Controlled Release: an Analytical Characterization

Overview

Journal J Anal Methods Chem

Publisher Wiley

Specialty Chemistry

Date 2015 Jan 24

PMID 25614850

Citations 7

Authors

Annalisa Bianchera

Enrico Salomi

Matteo Pezzanera

Elisabeth Ruwet

Ruggero Bettini

Lisa Elviri

Affiliations

Soon will be listed here.

Abstract

This paper provides an analytical characterization of chitosan scaffolds obtained by freeze-gelation toward the uptake and the controlled release of chondroitin sulphate (CS), as cartilage repair agent, under different pH conditions. Scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and liquid chromatography-UV spectrophotometry (LC-UV) techniques were exploited to obtain qualitative and quantitative descriptions of polymer and drug behaviour in the biomaterial. As for morphology, SEM analysis allowed the evaluation of scaffold porosity in terms of pore size and distribution both at the surface (Feret diameter 58 ± 19 μm) and on the cross section (Feret diameter 106 ± 51 μm). LC and ATR-FTIR evidenced a pH-dependent CS loading and release behaviour, strongly highlighting the role of electrostatic forces on chitosan/chondroitin sulphate interactions.

Citing Articles

Layer-by-layer assembly of nanotheranostic particles for simultaneous delivery of docetaxel and doxorubicin to target osteosarcoma.

Desmond L, Margini S, Barchiesi E, Pontrelli G, Phan A, Gentile P APL Bioeng. 2024; 8(1):016113.

PMID: 38445236 PMC: 10913103. DOI: 10.1063/5.0180831.

Extended-release of doxorubicin through green surface modification of gold nanoparticles: in vitro and in ovo assessment.

Asariha M, Kiaie S, Izadi S, H Pirhayati F, Fouladi M, Gholamhosseinpour M BMC Chem. 2022; 16(1):110.

PMID: 36474292 PMC: 9724295. DOI: 10.1186/s13065-022-00895-x.

New modular platform based on multi-adjuvanted amphiphilic chitosan nanoparticles for efficient lipopeptide vaccine delivery against group A streptococcus.

Mohamad Norpi A, Nordin M, Ahmad N, Katas H, Fuaad A, Sukri A Asian J Pharm Sci. 2022; 17(3):435-446.

PMID: 35782331 PMC: 9237632. DOI: 10.1016/j.ajps.2022.04.002.

Cultured Horse Articular Chondrocytes in 3D-Printed Chitosan Scaffold With Hyaluronic Acid and Platelet Lysate.

De Angelis E, Saleri R, Martelli P, Elviri L, Bianchera A, Bergonzi C Front Vet Sci. 2021; 8:671776.

PMID: 34322533 PMC: 8311290. DOI: 10.3389/fvets.2021.671776.

Novel Clove Essential Oil Nanoemulgel Tailored by Taguchi's Model and Scaffold-Based Nanofibers: Phytopharmaceuticals with Promising Potential as Cyclooxygenase-2 Inhibitors in External Inflammation.

Aman R, Abu Hashim I, Meshali M Int J Nanomedicine. 2020; 15:2171-2195.

PMID: 32280213 PMC: 7125334. DOI: 10.2147/IJN.S246601.

References

Pipitone V . Chondroprotection with chondroitin sulfate. Drugs Exp Clin Res. 1991; 17(1):3-7. View

Huang H, Zhang X, Hu X, Dai L, Zhu J, Man Z . Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering. Biomed Mater. 2014; 9(3):035008. DOI: 10.1088/1748-6041/9/3/035008. View

Varghese S, Theprungsirikul P, Sahani S, Hwang N, Yarema K, Elisseeff J . Glucosamine modulates chondrocyte proliferation, matrix synthesis, and gene expression. Osteoarthritis Cartilage. 2006; 15(1):59-68. DOI: 10.1016/j.joca.2006.06.008. View

Abarrategi A, Lopiz-Morales Y, Ramos V, Civantos A, Lopez-Duran L, Marco F . Chitosan scaffolds for osteochondral tissue regeneration. J Biomed Mater Res A. 2010; 95(4):1132-41. DOI: 10.1002/jbm.a.32912. View

Busilacchi A, Gigante A, Mattioli-Belmonte M, Manzotti S, Muzzarelli R . Chitosan stabilizes platelet growth factors and modulates stem cell differentiation toward tissue regeneration. Carbohydr Polym. 2013; 98(1):665-76. DOI: 10.1016/j.carbpol.2013.06.044. View

Sechriest V, Miao Y, Niyibizi C, Matthew H, Evans C, Fu F . GAG-augmented polysaccharide hydrogel: a novel biocompatible and biodegradable material to support chondrogenesis. J Biomed Mater Res. 1999; 49(4):534-41. DOI: 10.1002/(sici)1097-4636(20000315)49:4<534::aid-jbm12>3.0.co;2-#. View

Calamia V, Lourido L, Fernandez-Puente P, Mateos J, Rocha B, Montell E . Secretome analysis of chondroitin sulfate-treated chondrocytes reveals anti-angiogenic, anti-inflammatory and anti-catabolic properties. Arthritis Res Ther. 2012; 14(5):R202. PMC: 3580514. DOI: 10.1186/ar4040. View

Martel-Pelletier J, Kwan Tat S, Pelletier J . Effects of chondroitin sulfate in the pathophysiology of the osteoarthritic joint: a narrative review. Osteoarthritis Cartilage. 2010; 18 Suppl 1:S7-11. DOI: 10.1016/j.joca.2010.01.015. View

Park Y, Lee Y, Lee J, Seol Y, Chung C, Lee S . Controlled release of platelet-derived growth factor-BB from chondroitin sulfate-chitosan sponge for guided bone regeneration. J Control Release. 2000; 67(2-3):385-94. DOI: 10.1016/s0168-3659(00)00232-7. View

10.

Mi F, Shyu S, Peng C, Wu Y, Sung H, Wang P . Fabrication of chondroitin sulfate-chitosan composite artificial extracellular matrix for stabilization of fibroblast growth factor. J Biomed Mater Res A. 2005; 76(1):1-15. DOI: 10.1002/jbm.a.30298. View

11.

Ruffell B, Poon G, Lee S, Brown K, Tjew S, Cooper J . Differential use of chondroitin sulfate to regulate hyaluronan binding by receptor CD44 in Inflammatory and Interleukin 4-activated Macrophages. J Biol Chem. 2011; 286(22):19179-90. PMC: 3103297. DOI: 10.1074/jbc.M110.200790. View

12.

Schwartz Z, Griffon D, Fredericks L, Lee H, Weng H . Hyaluronic acid and chondrogenesis of murine bone marrow mesenchymal stem cells in chitosan sponges. Am J Vet Res. 2011; 72(1):42-50. DOI: 10.2460/ajvr.72.1.42. View

13.

Yeh M, Cheng K, Hu C, Huang Y, Young J . Novel protein-loaded chondroitin sulfate-chitosan nanoparticles: preparation and characterization. Acta Biomater. 2011; 7(10):3804-12. DOI: 10.1016/j.actbio.2011.06.026. View

14.

Johnson P, Ruffell B . CD44 and its role in inflammation and inflammatory diseases. Inflamm Allergy Drug Targets. 2009; 8(3):208-20. DOI: 10.2174/187152809788680994. View

15.

Lippiello L, Woodward J, Karpman R, Hammad T . In vivo chondroprotection and metabolic synergy of glucosamine and chondroitin sulfate. Clin Orthop Relat Res. 2000; (381):229-40. DOI: 10.1097/00003086-200012000-00027. View

16.

Henson F, Getgood A, Caborn D, McIlwraith C, Rushton N . Effect of a solution of hyaluronic acid-chondroitin sulfate-N-acetyl glucosamine on the repair response of cartilage to single-impact load damage. Am J Vet Res. 2012; 73(2):306-12. DOI: 10.2460/ajvr.73.2.306. View

17.

Muzzarelli R, Greco F, Busilacchi A, Sollazzo V, Gigante A . Chitosan, hyaluronan and chondroitin sulfate in tissue engineering for cartilage regeneration: a review. Carbohydr Polym. 2014; 89(3):723-39. DOI: 10.1016/j.carbpol.2012.04.057. View

18.

Schindeler A, Kolind M, Little D . Cellular transitions and tissue engineering. Cell Reprogram. 2013; 15(2):101-6. PMC: 3616450. DOI: 10.1089/cell.2012.0054. View

19.

Yagoubi N, Denuziere A, Pellerin F, Ferrier D . [Characterization and simultaneous assay of polymers and additives composing multilayer plastic material for pharmaceutical use by FTIR]. Ann Pharm Fr. 1996; 54(3):126-30. View

20.

Chen W, Wang L, Chen J, Fan S . Characterization of polyelectrolyte complexes between chondroitin sulfate and chitosan in the solid state. J Biomed Mater Res A. 2005; 75(1):128-37. DOI: 10.1002/jbm.a.30393. View