Anti-quorum Sensing Potential of Adenanthera Pavonina

Overview

Journal Pharmacognosy Res

Date 2015 Jan 20

PMID 25598643

Citations 6

Authors

Halkare Suryanarayana Vasavi

Ananthapadmanabha Bhagwath Arun

Punchappady-Devasya Rekha

Affiliations

Soon will be listed here.

Abstract

Background: Quorum sensing (QS) in Pseudomonas aeruginosa plays a key role in virulence factor production, biofilm formation and antimicrobial resistance. Because of emerging antimicrobial resistance in P. aeruginosa, there is a need to find an alternate nonantibiotic agent for the control of infections caused by this organism.

Objective: To evaluate anti-QS activity of Adenanthera pavonina L., a medicinal plant used in traditional medicine.

Materials And Methods: Preliminary screening for anti-QS activity of ethanol extract of A. pavonina was carried out using Chromobacterium violaceum CV026 biosensor strain and inhibition of QS-regulated violacein production was quantified using C. violaceum ATCC12472. Bioassay guided fractionation of ethanol extract resulted in ethyl acetate fraction (AEF) with strong anti-QS activity and AEF was evaluated for inhibition of QS-regulated pyocyanin production, proteolytic, elastolytic activity, swarming motility and biofilm formation in P. aeruginosa PAO1.

Results: AEF, at 0.5 mg/ml, inhibited pyocyanin production completely and at 1 mg/ml of AEF, complete inhibition of proteolytic and elastolytic activities were observed. However, viability of P. aeruginosa PAO1 was not affected at the tested concentrations of AEF as observed by cell count. Swarming motility was inhibited at the concentration of 0.1 mg/ml of AEF. Thin layer chromatography and biosensor overlay of AEF showed violacein inhibition zone at Rf value 0.63.

Conclusion: From the results of this study, it can be concluded that A. pavonina extracts can be used as effective anti-QS agents.

Citing Articles

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Antibacterial Activity and Anti-Quorum Sensing Mediated Phenotype in Response to Essential Oil from Leaves.

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Flavones as Quorum Sensing Inhibitors Identified by a Newly Optimized Screening Platform Using Chromobacterium violaceum as Reporter Bacteria.

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