Article: Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP

Designing Molecularly Imprinted Polymers for sensing proteins is still a somewhat empirical process due to the inherent complexity of protein imprinting. Based on Bovine Serum Albumin as a model analyte, we explored the influence of a range of experimental parameters on the final sensor responses. The optimized polymer contains 70% cross linker. Lower amounts lead to higher sensitivity, but also sensor response times substantially increase (to up to 10 h) at constant imprinting effect (signal ratio MIP/NIP on quartz crystal microbalance-QCM). However, by shifting the polymer properties to more hydrophilic by replacing methacrylic acid by acrylic acid, part of the decreased sensitivity can be recovered leading to appreciable sensor responses. Changing polymer morphology by bulk imprinting and nanoparticle approaches has much lower influence on sensitivity.

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References

1.

Bossi A, Bonini F, Turner A, Piletsky S . Molecularly imprinted polymers for the recognition of proteins: the state of the art. Biosens Bioelectron. 2006; 22(6):1131-7. DOI: 10.1016/j.bios.2006.06.023. View

2.

Cakir P, Cutivet A, Resmini M, Tse Sum Bui B, Haupt K . Protein-size molecularly imprinted polymer nanogels as synthetic antibodies, by localized polymerization with multi-initiators. Adv Mater. 2012; 25(7):1048-51. DOI: 10.1002/adma.201203400. View

3.

Seidler K, Lieberzeit P, Dickert F . Application of yeast imprinting in biotechnology and process control. Analyst. 2009; 134(2):361-6. DOI: 10.1039/b809130g. View

4.

Muratsugu M, OHTA F, Miya Y, Hosokawa T, Kurosawa S, Kamo N . Quartz crystal microbalance for the detection of microgram quantities of human serum albumin: relationship between the frequency change and the mass of protein adsorbed. Anal Chem. 1993; 65(20):2933-7. DOI: 10.1021/ac00068a036. View

5.

Turner N, Jeans C, Brain K, Allender C, Hlady V, Britt D . From 3D to 2D: a review of the molecular imprinting of proteins. Biotechnol Prog. 2006; 22(6):1474-89. PMC: 2666979. DOI: 10.1021/bp060122g. View

6.

Lieberzeit P, Rehman A, Najafi B, Dickert F . Real-life application of a QCM-based e-nose: quantitative characterization of different plant-degradation processes. Anal Bioanal Chem. 2008; 391(8):2897-903. DOI: 10.1007/s00216-008-2222-6. View

7.

Nopper D, Lammershop O, Wulff G, Gauglitz G . Amidine-based molecularly imprinted polymers-new sensitive elements for chiral chemosensors. Anal Bioanal Chem. 2003; 377(4):608-13. DOI: 10.1007/s00216-003-2145-1. View

8.

Alenus J, Ethirajan A, Horemans F, Weustenraed A, Csipai P, Gruber J . Molecularly imprinted polymers as synthetic receptors for the QCM-D-based detection of L-nicotine in diluted saliva and urine samples. Anal Bioanal Chem. 2013; 405(20):6479-87. DOI: 10.1007/s00216-013-7080-1. View

9.

Yoshimatsu K, Reimhult K, Krozer A, Mosbach K, Sode K, Ye L . Uniform molecularly imprinted microspheres and nanoparticles prepared by precipitation polymerization: the control of particle size suitable for different analytical applications. Anal Chim Acta. 2007; 584(1):112-21. DOI: 10.1016/j.aca.2006.11.004. View

10.

Rothschild M, ORATZ M, SCHREIBER S . Serum albumin. Hepatology. 1988; 8(2):385-401. DOI: 10.1002/hep.1840080234. View

11.

Poma A, Turner A, Piletsky S . Advances in the manufacture of MIP nanoparticles. Trends Biotechnol. 2010; 28(12):629-37. DOI: 10.1016/j.tibtech.2010.08.006. View