Altered Pulmonary Artery Endothelial-smooth Muscle Cell Interactions in Experimental Congenital Diaphragmatic Hernia

Overview

Journal Pediatr Res

Specialties Biology
Pediatrics

Date 2015 Jan 13

PMID 25580737

Citations 11

Authors

Shannon N Acker

Gregory J Seedorf

Steven H Abman

Eva Nozik-Grayck

Katherine Kuhn

David A Partrick

Jason Gien

Affiliations

Soon will be listed here.

Abstract

Background: Pulmonary hypertension (PH) secondary to vascular remodeling contributes to poor outcomes in congenital diaphragmatic hernia (CDH), however mechanisms responsible are unknown. We hypothesized that pulmonary artery endothelial cell (PAEC) dysfunction contributes to smooth muscle cell (SMC) hyperplasia in experimental CDH.

Methods: PAEC and SMC were isolated from fetal sheep with experimental CDH and controls. SMC growth was assessed alone and with SOD plus catalase and during coculture with control or CDH PAEC with and without ET-1 siRNA transfection. ET-1 protein was measured in PAEC and SMC lysates and supernatant. ROS production was measured in normal and CDH PAECs with and without ET-1 siRNA. PAEC growth and tube formation were measured with SOD plus catalase.

Results: CDH SMC growth was decreased and increased with coculture with CDH PAEC more than control PAEC. Treatment of CDH PAEC with SOD plus catalase or ET-1 siRNA prevented the increase in SMC growth seen with coculture. ET-1 protein was increased in CDH PAEC and SMC. ROS production was increased in CDH PAEC and decreased with ET-1 SiRNA. SOD plus catalase restored CDH PAEC growth and tube formation.

Conclusion: PAEC dysfunction in experimental CDH increases SMC proliferation via ET-1 induced ROS production by PAEC.

Citing Articles

Hemodynamic management of congenital diaphragmatic hernia: the role of targeted neonatal echocardiography.

Surak A, Mahgoub L, Ting J World J Pediatr Surg. 2024; 7(2):e000790.

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Single-cell transcriptomic profiling of microvascular endothelial cell heterogeneity in congenital diaphragmatic hernia.

Robertson J, Bazeley P, Erzurum S, Asosingh K Sci Rep. 2023; 13(1):9851.

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Effect of prenatal diaphragmatic hernia on pulmonary arterial morphology.

Stainsby A, DeKoninck P, Crossley K, Thiel A, Wallace M, Pearson J Anat Rec (Hoboken). 2023; 308(4):1082-1093.

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Neonatal Selenium Deficiency Decreases Selenoproteins in the Lung and Impairs Pulmonary Alveolar Development.

Sherlock L, McCarthy W, Grayck M, Solar M, Hernandez A, Zheng L Antioxidants (Basel). 2022; 11(12).

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Cellular, molecular, and metabolic aspects of developing lungs in congenital diaphragmatic hernia.

Perveen S, Frigeni M, Benveniste H, Kurepa D Front Pediatr. 2022; 10:932463.

PMID: 36458148 PMC: 9706094. DOI: 10.3389/fped.2022.932463.

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