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Translatome Analyses Capture of Opposing Tissue-specific Brassinosteroid Signals Orchestrating Root Meristem Differentiation

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Specialty Science
Date 2015 Jan 7
PMID 25561530
Citations 57
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Abstract

The mechanisms ensuring balanced growth remain a critical question in developmental biology. In plants, this balance relies on spatiotemporal integration of hormonal signaling pathways, but the understanding of the precise contribution of each hormone is just beginning to take form. Brassinosteroid (BR) hormone is shown here to have opposing effects on root meristem size, depending on its site of action. BR is demonstrated to both delay and promote onset of stem cell daughter differentiation, when acting in the outer tissue of the root meristem, the epidermis, and the innermost tissue, the stele, respectively. To understand the molecular basis of this phenomenon, a comprehensive spatiotemporal translatome mapping of Arabidopsis roots was performed. Analyses of wild type and mutants featuring different distributions of BR revealed autonomous, tissue-specific gene responses to BR, implying its contrasting tissue-dependent impact on growth. BR-induced genes were primarily detected in epidermal cells of the basal meristem zone and were enriched by auxin-related genes. In contrast, repressed BR genes prevailed in the stele of the apical meristem zone. Furthermore, auxin was found to mediate the growth-promoting impact of BR signaling originating in the epidermis, whereas BR signaling in the stele buffered this effect. We propose that context-specific BR activity and responses are oppositely interpreted at the organ level, ensuring coherent growth.

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References
1.
Chung Y, Maharjan P, Lee O, Fujioka S, Jang S, Kim B . Auxin stimulates DWARF4 expression and brassinosteroid biosynthesis in Arabidopsis. Plant J. 2011; 66(4):564-78. DOI: 10.1111/j.1365-313X.2011.04513.x. View

2.
Jaillais Y, Hothorn M, Belkhadir Y, Dabi T, Nimchuk Z, Meyerowitz E . Tyrosine phosphorylation controls brassinosteroid receptor activation by triggering membrane release of its kinase inhibitor. Genes Dev. 2011; 25(3):232-7. PMC: 3034898. DOI: 10.1101/gad.2001911. View

3.
Stepanova A, Robertson-Hoyt J, Yun J, Benavente L, Xie D, Dolezal K . TAA1-mediated auxin biosynthesis is essential for hormone crosstalk and plant development. Cell. 2008; 133(1):177-91. DOI: 10.1016/j.cell.2008.01.047. View

4.
Liu J, Sheng L, Xu Y, Li J, Yang Z, Huang H . WOX11 and 12 are involved in the first-step cell fate transition during de novo root organogenesis in Arabidopsis. Plant Cell. 2014; 26(3):1081-93. PMC: 4001370. DOI: 10.1105/tpc.114.122887. View

5.
Ubeda-Tomas S, Beemster G, Bennett M . Hormonal regulation of root growth: integrating local activities into global behaviour. Trends Plant Sci. 2012; 17(6):326-31. DOI: 10.1016/j.tplants.2012.02.002. View