Isolation and Characterization of Oxaloacetate Decarboxylase of Salmonella Typhimurium, a Sodium Ion Pump

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 1989 Jan 1

PMID 2556085

Citations 13

Authors

K Wifling

P Dimroth

Affiliations

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Abstract

Anaerobic growth of Salmonella typhimurium on citrate is Na+-dependent and requires induction of the necessary enzymes during a 20-40 h lag phase. The citrate fermentation pathway involves citrate lyase and oxaloacetate decarboxylase. The decarboxylase is a membrane-bound, Na+-activated, biotin-containing enzyme that functions as a Na+ pump. Oxaloacetate decarboxylase was isolated by affinity chromatography of a Triton X-100 extract of the bacterial membranes on avidin-Sepharose. The enzyme consists of three subunits alpha, beta, gamma, with apparent molecular weights of 63,800, 34,500 and 10,600. The alpha-chain contains a covalently attached biotin group and binds to antibodies raised against the alpha-subunit of oxaloacetate decarboxylase from Klebsiella pneumoniae. The Na+ transport function was reconstituted by incorporation of the purified enzyme into proteoliposomes.

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References

Dimroth P, Thomer A . A primary respiratory Na+ pump of an anaerobic bacterium: the Na+-dependent NADH:quinone oxidoreductase of Klebsiella pneumoniae. Arch Microbiol. 1989; 151(5):439-44. DOI: 10.1007/BF00416604. View

Dimroth P, Thomer A . Subunit composition of oxaloacetate decarboxylase and characterization of the alpha chain as carboxyltransferase. Eur J Biochem. 1983; 137(1-2):107-12. DOI: 10.1111/j.1432-1033.1983.tb07802.x. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

OBrien R, Frost G, Stern J . Enzymatic analysis of the requirement for sodium in aerobic growth of Salmonella typhimurium on citrate. J Bacteriol. 1969; 99(2):395-400. PMC: 250029. DOI: 10.1128/jb.99.2.395-400.1969. View

Tsang V, Peralta J, Simons A . Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol. 1983; 92:377-91. DOI: 10.1016/0076-6879(83)92032-3. View

Kroger A . Electron-transport phosphorylation coupled to fumarate reduction in anaerobically grown Proteus rettgeri. Biochim Biophys Acta. 1974; 347(2):273-89. DOI: 10.1016/0005-2728(74)90051-6. View

Dimroth P, Thomer A . Citrate transport in Klebsiella pneumoniae. Biol Chem Hoppe Seyler. 1986; 367(8):813-23. DOI: 10.1515/bchm3.1986.367.2.813. View

DAGLEY S . Dissimilation of citric acid by Aerobacter aerogenes and Escherichia coli. J Gen Microbiol. 1954; 11(2):218-27. DOI: 10.1099/00221287-11-2-218. View

Lutgens M, Gottschalk G . Why a co-substrate is required for anaerobic growth of Escherichia coli on citrate. J Gen Microbiol. 1980; 119(1):63-70. DOI: 10.1099/00221287-119-1-63. View

10.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

11.

Laussermair E, Schwarz E, Oesterhelt D, Reinke H, Beyreuther K, Dimroth P . The sodium ion translocating oxaloacetate decarboxylase of Klebsiella pneumoniae. Sequence of the integral membrane-bound subunits beta and gamma. J Biol Chem. 1989; 264(25):14710-5. View

12.

Stern J . Oxalacetate decarboxylase of Aerobacter aerogenes. I. Inhibition by avidin and requirement for sodium ion. Biochemistry. 1967; 6(11):3545-51. DOI: 10.1021/bi00863a028. View

13.

KAY W, Cameron M . Citrate transport in Salmonella typhimurium. Arch Biochem Biophys. 1978; 190(1):270-80. DOI: 10.1016/0003-9861(78)90276-x. View

14.

Schwarz E, Oesterhelt D, Reinke H, Beyreuther K, Dimroth P . The sodium ion translocating oxalacetate decarboxylase of Klebsiella pneumoniae. Sequence of the biotin-containing alpha-subunit and relationship to other biotin-containing enzymes. J Biol Chem. 1988; 263(20):9640-5. View

15.

Dimroth P . Preparation, characterization, and reconstitution of oxaloacetate decarboxylase from Klebsiella aerogenes, a sodium pump. Methods Enzymol. 1986; 125:530-40. DOI: 10.1016/s0076-6879(86)25042-9. View

16.

AMARASINGHAM C, Davis B . Regulation of alpha-ketoglutarate dehydrogenase formation in Escherichia coli. J Biol Chem. 1965; 240(9):3664-8. View

17.

Dimroth P, Thomer A . Dissociation of the sodium-ion-translocating oxaloacetate decarboxylase of Klebsiella pneumoniae and reconstitution of the active complex from the isolated subunits. Eur J Biochem. 1988; 175(1):175-80. DOI: 10.1111/j.1432-1033.1988.tb14180.x. View

18.

Dimroth P . The role of biotin and sodium in the decarboxylation of oxaloacetate by the membrane-bound oxaloacetate decarboxylase from Klebsiella aerogenes. Eur J Biochem. 1982; 121(2):435-41. DOI: 10.1111/j.1432-1033.1982.tb05806.x. View

19.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

20.

Hilpert W, Dimroth P . Purification and characterization of a new sodium-transport decarboxylase. Methylmalonyl-CoA decarboxylase from Veillonella alcalescens. Eur J Biochem. 1983; 132(3):579-87. DOI: 10.1111/j.1432-1033.1983.tb07403.x. View