MicroRNA and Messenger RNA Profiling Reveals New Biomarkers and Mechanisms for RDX Induced Neurotoxicity

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2015 Jan 7

PMID 25559034

Citations 10

Authors

Youping Deng

Junmei Ai

Xin Guan

Zhaohui Wang

Bin Yan

Daqin Zhang

Chang Liu

Mitch S Wilbanks

Barbara Lynn Escalon

Sharon A Meyers

Mary Qu Yang

Edward J Perkins

Affiliations

Soon will be listed here.

Abstract

Background: RDX is a well-known pollutant to induce neurotoxicity. MicroRNAs (miRNA) and messenger RNA (mRNA) profiles are useful tools for toxicogenomics studies. It is worthy to integrate MiRNA and mRNA expression data to understand RDX-induced neurotoxicity.

Results: Rats were treated with or without RDX for 48 h. Both miRNA and mRNA profiles were conducted using brain tissues. Nine miRNAs were significantly regulated by RDX. Of these, 6 and 3 miRNAs were up- and down-regulated respectively. The putative target genes of RDX-regulated miRNAs were highly nervous system function genes and pathways enriched. Fifteen differentially genes altered by RDX from mRNA profiles were the putative targets of regulated miRNAs. The induction of miR-71, miR-27ab, miR-98, and miR-135a expression by RDX, could reduce the expression of the genes POLE4, C5ORF13, SULF1 and ROCK2, and eventually induce neurotoxicity. Over-expression of miR-27ab, or reduction of the expression of unknown miRNAs by RDX, could up-regulate HMGCR expression and contribute to neurotoxicity. RDX regulated immune and inflammation response miRNAs and genes could contribute to RDX- induced neurotoxicity and other toxicities as well as animal defending reaction response to RDX exposure.

Conclusions: Our results demonstrate that integrating miRNA and mRNA profiles is valuable to indentify novel biomarkers and molecular mechanisms for RDX-induced neurological disorder and neurotoxicity.

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