A Negative Regulatory Element in the Promoter of the Human Alpha 1-antitrypsin Gene

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1989 Nov 25

PMID 2555792

Citations 5

Authors

V De Simone

R Cortese

Affiliations

Soon will be listed here.

Abstract

A "minimal tissue-specific element" of the human alpha 1-antitrypsin (alpha 1-AT) gene promoter, located in the -137/-37 region, is able to drive hepatoma-specific transcription from the heterologous SV40 promoter. Here we show that, in HeLa cells, this element is inactivated by a negative regulatory mechanism. This negative control can be overcome by deletion or mutation of two specific DNA domains.

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References

Peritz L, Fodor E, Silversides D, Cattini P, Baxter J, Eberhardt N . The human growth hormone gene contains both positive and negative control elements. J Biol Chem. 1988; 263(11):5005-7. View

Ehrlich R, Maguire J, Singer D . Identification of negative and positive regulatory elements associated with a class I major histocompatibility complex gene. Mol Cell Biol. 1988; 8(2):695-703. PMC: 363195. DOI: 10.1128/mcb.8.2.695-703.1988. View

Reue K, Leff T, Breslow J . Human apolipoprotein CIII gene expression is regulated by positive and negative cis-acting elements and tissue-specific protein factors. J Biol Chem. 1988; 263(14):6857-64. View

Das H, Leff T, Breslow J . Cell type-specific expression of the human apoB gene is controlled by two cis-acting regulatory regions. J Biol Chem. 1988; 263(23):11452-8. View

Monaci P, Nicosia A, Cortese R . Two different liver-specific factors stimulate in vitro transcription from the human alpha 1-antitrypsin promoter. EMBO J. 1988; 7(7):2075-87. PMC: 454491. DOI: 10.1002/j.1460-2075.1988.tb03047.x. View

Hardon E, Frain M, Paonessa G, Cortese R . Two distinct factors interact with the promoter regions of several liver-specific genes. EMBO J. 1988; 7(6):1711-9. PMC: 457158. DOI: 10.1002/j.1460-2075.1988.tb03000.x. View

De Simone V, Cortese R . The transcriptional regulation of liver-specific gene expression. Oxf Surv Eukaryot Genes. 1988; 5:51-90. View

Graham F, Van Der Eb A . A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973; 52(2):456-67. DOI: 10.1016/0042-6822(73)90341-3. View

Berk A, Sharp P . Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977; 12(3):721-32. DOI: 10.1016/0092-8674(77)90272-0. View

10.

Chirgwin J, Przybyla A, MacDonald R, Rutter W . Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979; 18(24):5294-9. DOI: 10.1021/bi00591a005. View

11.

Knowles B, HOWE C, Aden D . Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science. 1980; 209(4455):497-9. DOI: 10.1126/science.6248960. View

12.

Gorman C, Merlino G, Willingham M, Pastan I, Howard B . The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc Natl Acad Sci U S A. 1982; 79(22):6777-81. PMC: 347216. DOI: 10.1073/pnas.79.22.6777. View

13.

Wasylyk B, Wasylyk C, Chambon P . Short and long range activation by the SV40 enhancer. Nucleic Acids Res. 1984; 12(14):5589-608. PMC: 320017. DOI: 10.1093/nar/12.14.5589. View

14.

Donofrio C, Colantuoni V, Cortese R . Structure and cell-specific expression of a cloned human retinol binding protein gene: the 5'-flanking region contains hepatoma specific transcriptional signals. EMBO J. 1985; 4(8):1981-9. PMC: 554451. DOI: 10.1002/j.1460-2075.1985.tb03881.x. View

15.

DE Wet J, Wood K, DeLuca M, Helinski D, Subramani S . Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol. 1987; 7(2):725-37. PMC: 365129. DOI: 10.1128/mcb.7.2.725-737.1987. View

16.

Greenberg A, Ber R, Kra-Oz Z, LASKOV R . Extinction of expression of immunoglobulin genes in myeloma X fibroblast somatic cell hybrids. Mol Cell Biol. 1987; 7(2):936-9. PMC: 365154. DOI: 10.1128/mcb.7.2.936-939.1987. View

17.

Chin A, Fournier R . A genetic analysis of extinction: trans-regulation of 16 liver-specific genes in hepatoma-fibroblast hybrid cells. Proc Natl Acad Sci U S A. 1987; 84(6):1614-8. PMC: 304486. DOI: 10.1073/pnas.84.6.1614. View

18.

Imler J, Lemaire C, Wasylyk C, Wasylyk B . Negative regulation contributes to tissue specificity of the immunoglobulin heavy-chain enhancer. Mol Cell Biol. 1987; 7(7):2558-67. PMC: 365391. DOI: 10.1128/mcb.7.7.2558-2567.1987. View

19.

Baniahmad A, Muller M, Steiner C, Renkawitz R . Activity of two different silencer elements of the chicken lysozyme gene can be compensated by enhancer elements. EMBO J. 1987; 6(8):2297-303. PMC: 553632. DOI: 10.1002/j.1460-2075.1987.tb02504.x. View

20.

Gaub M, Dierich A, Astinotti D, Touitou I, Chambon P . The chicken ovalbumin promoter is under negative control which is relieved by steroid hormones. EMBO J. 1987; 6(8):2313-20. PMC: 553634. DOI: 10.1002/j.1460-2075.1987.tb02506.x. View