Properties of the DNA-binding Domain of the Simian Virus 40 Large T Antigen

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1989 Dec 1

PMID 2555700

Citations 18

Authors

D McVey

M Strauss

Y Gluzman

Affiliations

Soon will be listed here.

Abstract

T antigen (Tag) from simian virus 40 binds specifically to two distinct sites in the viral origin of replication and to single-stranded DNA. Analysis of the protein domain responsible for these activities revealed the following. (i) The C-terminal boundary of the origin-specific and single-strand-specific DNA-binding domain is at or near amino acid 246; furthermore, the maximum of these DNA-binding activities coincides with a narrow C-terminal boundary, spanning 4 amino acids (246 to 249) and declines sharply in proteins with C termini which differ by a few (4 to 10) amino acids; (ii) a polypeptide spanning residues 132 to 246 of Tag is an independent domain responsible for origin-specific DNA binding and presumably for single-stranded DNA binding; and (iii) a comparison of identical N-terminal fragments of Tag purified from mammalian and bacterial cells revealed differential specificity and levels of activity between the two sources of protein. A role for posttranslational modification (phosphorylation) in controlling the DNA-binding activity of Tag is discussed.

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References

Evans R, Hollenberg S . Zinc fingers: gilt by association. Cell. 1988; 52(1):1-3. DOI: 10.1016/0092-8674(88)90522-3. View

Klausing K, Scheidtmann K, BAUMANN E, Knippers R . Effects of in vitro dephosphorylation on DNA-binding and DNA helicase activities of simian virus 40 large tumor antigen. J Virol. 1988; 62(4):1258-65. PMC: 253135. DOI: 10.1128/JVI.62.4.1258-1265.1988. View

Mole S, Gannon J, Ford M, Lane D . Structure and function of SV40 large-T antigen. Philos Trans R Soc Lond B Biol Sci. 1987; 317(1187):455-69. DOI: 10.1098/rstb.1987.0072. View

Simmons D . Geometry of the simian virus 40 large tumor antigen-DNA complex as probed by protease digestion. Proc Natl Acad Sci U S A. 1988; 85(7):2086-90. PMC: 279933. DOI: 10.1073/pnas.85.7.2086. View

Arthur A, Hoss A, Fanning E . Expression of simian virus 40 T antigen in Escherichia coli: localization of T-antigen origin DNA-binding domain to within 129 amino acids. J Virol. 1988; 62(6):1999-2006. PMC: 253284. DOI: 10.1128/JVI.62.6.1999-2006.1988. View

Auborn K, Markowitz R, Wang E, Yu Y, Prives C . Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences. J Virol. 1988; 62(6):2204-8. PMC: 253332. DOI: 10.1128/JVI.62.6.2204-2208.1988. View

Borowiec J, Hurwitz J . Localized melting and structural changes in the SV40 origin of replication induced by T-antigen. EMBO J. 1988; 7(10):3149-58. PMC: 454705. DOI: 10.1002/j.1460-2075.1988.tb03182.x. View

Loeber G, Parsons R, Tegtmeyer P . The zinc finger region of simian virus 40 large T antigen. J Virol. 1989; 63(1):94-100. PMC: 247661. DOI: 10.1128/JVI.63.1.94-100.1989. View

Mohr I, Stillman B, Gluzman Y . Regulation of SV40 DNA replication by phosphorylation of T antigen. EMBO J. 1987; 6(1):153-60. PMC: 553371. DOI: 10.1002/j.1460-2075.1987.tb04733.x. View

10.

Wold M, Li J, Kelly T . Initiation of simian virus 40 DNA replication in vitro: large-tumor-antigen- and origin-dependent unwinding of the template. Proc Natl Acad Sci U S A. 1987; 84(11):3643-7. PMC: 304931. DOI: 10.1073/pnas.84.11.3643. View

11.

Bradley M, Smith T, Lathrop R, Livingston D, Webster T . Consensus topography in the ATP binding site of the simian virus 40 and polyomavirus large tumor antigens. Proc Natl Acad Sci U S A. 1987; 84(12):4026-30. PMC: 305014. DOI: 10.1073/pnas.84.12.4026. View

12.

Gish W, Botchan M . Simian virus 40-transformed human cells that express large T antigens defective for viral DNA replication. J Virol. 1987; 61(9):2864-76. PMC: 255807. DOI: 10.1128/JVI.61.9.2864-2876.1987. View

13.

Kadonaga J, Courey A, Ladika J, Tjian R . Distinct regions of Sp1 modulate DNA binding and transcriptional activation. Science. 1988; 242(4885):1566-70. DOI: 10.1126/science.3059495. View

14.

Auborn K, Guo M, Prives C . Helicase, DNA-binding, and immunological properties of replication-defective simian virus 40 mutant T antigens. J Virol. 1989; 63(2):912-8. PMC: 247765. DOI: 10.1128/JVI.63.2.912-918.1989. View

15.

Hoey T, Warrior R, Manak J, Levine M . DNA-binding activities of the Drosophila melanogaster even-skipped protein are mediated by its homeo domain and influenced by protein context. Mol Cell Biol. 1988; 8(11):4598-607. PMC: 365548. DOI: 10.1128/mcb.8.11.4598-4607.1988. View

16.

Otting G, Qian Y, Muller M, Affolter M, Gehring W, Wuthrich K . Secondary structure determination for the Antennapedia homeodomain by nuclear magnetic resonance and evidence for a helix-turn-helix motif. EMBO J. 1988; 7(13):4305-9. PMC: 455146. DOI: 10.1002/j.1460-2075.1988.tb03329.x. View

17.

Klausing K, Scheffner M, Scheidtmann K, Stahl H, Knippers R . DNA binding properties and replication activity of the T antigen related D2 phosphoprotein. Biochemistry. 1989; 28(5):2238-44. DOI: 10.1021/bi00431a040. View

18.

Mohr I, Gluzman Y, Fairman M, Strauss M, McVey D, Stillman B . Production of simian virus 40 large tumor antigen in bacteria: altered DNA-binding specificity and dna-replication activity of underphosphorylated large tumor antigen. Proc Natl Acad Sci U S A. 1989; 86(17):6479-83. PMC: 297867. DOI: 10.1073/pnas.86.17.6479. View

19.

Mohr I, Fairman M, Stillman B, Gluzman Y . Large T-antigen mutants define multiple steps in the initiation of simian virus 40 DNA replication. J Virol. 1989; 63(10):4181-8. PMC: 251032. DOI: 10.1128/JVI.63.10.4181-4188.1989. View

20.

Hansen U, Tenen D, Livingston D, Sharp P . T antigen repression of SV40 early transcription from two promoters. Cell. 1981; 27(3 Pt 2):603-13. DOI: 10.1016/0092-8674(81)90402-5. View