Infectious CDNA Clones of the DA Strain of Theiler's Murine Encephalomyelitis Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1989 Dec 1

PMID 2555569

Citations 50

Authors

R P Roos

S Stein

Y Ohara

J L Fu

B L Semler

Affiliations

Soon will be listed here.

Abstract

The DA strain and other members of the TO subgroup of Theiler's murine encephalomyelitis viruses cause a persistent demyelinating infection, whereas the GDVII strain and other GDVII subgroup strains cause an acute lethal polioencephalomyelitis. We generated an infectious DA cDNA clone inserted into a transcription vector. Virus derived from transfection of transcripts produced a demyelinating disease indistinguishable from that of wild-type virus. The infectious clone provides a critical reagent for the production of interstrain recombinant viruses to help identify genetic loci responsible for the biological activities of the strains.

Citing Articles

An elite controller of picornavirus infection targets an epitope that is resistant to immune escape.

Bell M, Renner D, Johnson A, Pavelko K PLoS One. 2014; 9(4):e94332.

PMID: 24710606 PMC: 3978045. DOI: 10.1371/journal.pone.0094332.

The epitope integration site for vaccine antigens determines virus control while maintaining efficacy in an engineered cancer vaccine.

Pavelko K, Bell M, Karyampudi L, Hansen M, Allen K, Knutson K Mol Ther. 2013; 21(5):1087-95.

PMID: 23568262 PMC: 3666639. DOI: 10.1038/mt.2013.52.

Immunization with structural and non-structural proteins of Theiler's murine encephalomyelitis virus alters demyelinating disease.

Tsunoda I, Libbey J, Fujinami R J Neurovirol. 2012; 18(2):127-37.

PMID: 22403027 PMC: 3335973. DOI: 10.1007/s13365-012-0087-0.

Saffold virus, a novel human Cardiovirus with unknown pathogenicity.

Himeda T, Ohara Y J Virol. 2011; 86(3):1292-6.

PMID: 22114344 PMC: 3264344. DOI: 10.1128/JVI.06087-11.

Theiler's murine encephalomyelitis virus as a vaccine candidate for immunotherapy.

Pavelko K, Girtman M, Mitsunaga Y, Mendez-Fernandez Y, Bell M, Hansen M PLoS One. 2011; 6(5):e20217.

PMID: 21625449 PMC: 3098865. DOI: 10.1371/journal.pone.0020217.

References

Lipton H . Persistent Theiler's murine encephalomyelitis virus infection in mice depends on plaque size. J Gen Virol. 1980; 46(1):169-77. DOI: 10.1099/0022-1317-46-1-169. View

Lorch Y, Friedmann A, Lipton H, Kotler M . Theiler's murine encephalomyelitis virus group includes two distinct genetic subgroups that differ pathologically and biologically. J Virol. 1981; 40(2):560-7. PMC: 256659. DOI: 10.1128/JVI.40.2.560-567.1981. View

Nitayaphan S, Toth M, Roos R . Neutralizing monoclonal antibodies to Theiler's murine encephalomyelitis viruses. J Virol. 1985; 53(2):651-7. PMC: 254681. DOI: 10.1128/JVI.53.2.651-657.1985. View

La Monica N, Meriam C, Racaniello V . Mapping of sequences required for mouse neurovirulence of poliovirus type 2 Lansing. J Virol. 1986; 57(2):515-25. PMC: 252764. DOI: 10.1128/JVI.57.2.515-525.1986. View

van der Werf S, Bradley J, Wimmer E, Studier F, Dunn J . Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase. Proc Natl Acad Sci U S A. 1986; 83(8):2330-4. PMC: 323290. DOI: 10.1073/pnas.83.8.2330. View

Semler B . In vitro molecular genetics as a tool for determining the differential cleavage specificities of the poliovirus 3C proteinase. Nucleic Acids Res. 1987; 15(5):2069-88. PMC: 340618. DOI: 10.1093/nar/15.5.2069. View

Roos R, Kong W, Semler B . Polyprotein processing of Theiler's murine encephalomyelitis virus. J Virol. 1989; 63(12):5344-53. PMC: 251201. DOI: 10.1128/JVI.63.12.5344-5353.1989. View

Ohara Y, Stein S, Fu J, Stillman L, Klaman L, Roos R . Molecular cloning and sequence determination of DA strain of Theiler's murine encephalomyelitis viruses. Virology. 1988; 164(1):245-55. DOI: 10.1016/0042-6822(88)90642-3. View

Ohara Y, Senkowski A, Fu J, Klaman L, Goodall J, Toth M . Trypsin-sensitive neutralization site on VP1 of Theiler's murine encephalomyelitis viruses. J Virol. 1988; 62(9):3527-9. PMC: 253483. DOI: 10.1128/JVI.62.9.3527-3529.1988. View

10.

Tangy F, Mcallister A, Brahic M . Molecular cloning of the complete genome of strain GDVII of Theiler's virus and production of infectious transcripts. J Virol. 1989; 63(3):1101-6. PMC: 247804. DOI: 10.1128/JVI.63.3.1101-1106.1989. View

11.

Ohara Y, Roos R . Viral infectious complementary-DNA studies may identify nonviral genes critical to central nervous system disease. Ann Neurol. 1989; 25(3):305-9. DOI: 10.1002/ana.410250316. View

12.

Roos R, Stein S, Routbort M, Senkowski A, Bodwell T, Wollmann R . Theiler's murine encephalomyelitis virus neutralization escape mutants have a change in disease phenotype. J Virol. 1989; 63(10):4469-73. PMC: 251072. DOI: 10.1128/JVI.63.10.4469-4473.1989. View

13.

Ohara Y, Roos R . The antibody response in Theiler's virus infection: new perspectives on multiple sclerosis. Prog Med Virol. 1987; 34:156-79. View