On-chip Multi-gas Incubation for Microfluidic Cell Cultures Under Hypoxia

Overview

Journal Biomicrofluidics

Publisher American Institute of Physics

Specialty Biomedical Engineering

Date 2015 Jan 2

PMID 25553177

Citations 3

Authors

Atsushi Takano

Masato Tanaka

Nobuyuki Futai

Affiliations

Soon will be listed here.

Abstract

We developed a simple system that regulates CO2 and O2 levels within a microfluidic chip. This system enables long-term cell culture under hypoxic conditions without the need of a CO2 incubator or a multi-gas incubator. Hypoxic conditions were generated using a miniature water jacket containing dissolved ascorbate as an oxygen scavenger. Formulations of the water jacket were determined that enables both 5% pCO2 and desired pO2 levels ranging from 5 to 15%. We also cultured PC-12 cells and primary neuronal cells from chick embryos under hypoxia and observed hypoxia-induced cell death and inhibition of neurite outgrowth.

Citing Articles

Integrated On-Chip 3D Vascular Network Culture under Hypoxia.

Olmedo-Suarez M, Sekiguchi T, Takano A, Canizares-Macias M, Futai N Micromachines (Basel). 2020; 11(5).

PMID: 32365889 PMC: 7281659. DOI: 10.3390/mi11050475.

Fabrication of composite microfluidic devices for local control of oxygen tension in cell cultures.

Gao Y, Stybayeva G, Revzin A Lab Chip. 2018; 19(2):306-315.

PMID: 30547179 PMC: 9555225. DOI: 10.1039/c8lc00825f.

Microfluidic Platform for the Long-Term On-Chip Cultivation of Mammalian Cells for Lab-On-A-Chip Applications.

Bunge F, van den Driesche S, Vellekoop M Sensors (Basel). 2017; 17(7).

PMID: 28698531 PMC: 5539486. DOI: 10.3390/s17071603.

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