Nitric Oxide Negatively Regulates Abscisic Acid Signaling in Guard Cells by S-nitrosylation of OST1

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2015 Jan 1

PMID 25550508

Citations 118

Authors

Pengcheng Wang

Yanyan Du

Yueh-Ju Hou

Yang Zhao

Chuan-Chih Hsu

Feijuan Yuan

Xiaohong Zhu

W Andy Tao

Chun-Peng Song

Jian-Kang Zhu

Affiliations

Soon will be listed here.

Abstract

The phytohormone abscisic acid (ABA) plays important roles in plant development and adaptation to environmental stress. ABA induces the production of nitric oxide (NO) in guard cells, but how NO regulates ABA signaling is not understood. Here, we show that NO negatively regulates ABA signaling in guard cells by inhibiting open stomata 1 (OST1)/sucrose nonfermenting 1 (SNF1)-related protein kinase 2.6 (SnRK2.6) through S-nitrosylation. We found that SnRK2.6 is S-nitrosylated at cysteine 137, a residue adjacent to the kinase catalytic site. Dysfunction in the S-nitrosoglutathione (GSNO) reductase (GSNOR) gene in the gsnor1-3 mutant causes NO overaccumulation in guard cells, constitutive S-nitrosylation of SnRK2.6, and impairment of ABA-induced stomatal closure. Introduction of the Cys137 to Ser mutated SnRK2.6 into the gsnor1-3/ost1-3 double-mutant partially suppressed the effect of gsnor1-3 on ABA-induced stomatal closure. A cysteine residue corresponding to Cys137 of SnRK2.6 is present in several yeast and human protein kinases and can be S-nitrosylated, suggesting that the S-nitrosylation may be an evolutionarily conserved mechanism for protein kinase regulation.

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