Automatic Cell Counting with ImageJ

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 2014 Dec 28

PMID 25542972

Citations 80

Authors

Ivan V Grishagin

Affiliations

Soon will be listed here.

Abstract

Cell counting is an important routine procedure. However, to date there is no comprehensive, easy to use, and inexpensive solution for routine cell counting, and this procedure usually needs to be performed manually. Here, we report a complete solution for automatic cell counting in which a conventional light microscope is equipped with a web camera to obtain images of a suspension of mammalian cells in a hemocytometer assembly. Based on the ImageJ toolbox, we devised two algorithms to automatically count these cells. This approach is approximately 10 times faster and yields more reliable and consistent results compared with manual counting.

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