A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus Comatus

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2014 Dec 26

PMID 25540778

Citations 9

Authors

Shuang Zhao

Cheng-Bo Rong

Chang Kong

Yu Liu

Feng Xu

Qian-Jiang Miao

Shou-Xian Wang

He-Xiang Wang

Guo-Qing Zhang

Affiliations

Soon will be listed here.

Abstract

A novel laccase was isolated and purified from fermentation mycelia of mushroom Coprinus comatus with an isolation procedure including three ion-exchange chromatography steps on DEAE-cellulose, CM-cellulose, and Q-Sepharose and one gel-filtration step by fast protein liquid chromatography on Superdex 75. The purified enzyme was a monomeric protein with a molecular weight of 64 kDa. It possessed a unique N-terminal amino acid sequence of AIGPVADLKV, which has considerably high sequence similarity with that of other fungal laccases, but is different from that of C. comatus laccases reported. The enzyme manifested an optimal pH value of 2.0 and an optimal temperature of 60°C using 2,2'-azinobis(3-ethylbenzothiazolone-6-sulfonic acid) diammonium salt (ABTS) as the substrate. The laccase displayed, at pH 2.0 and 37°C, K(m) values of 1.59 mM towards ABTS. It potently suppressed proliferation of tumor cell lines HepG2 and MCF7, and inhibited human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) with an IC50 value of 3.46 μM, 4.95 μM, and 5.85 μM, respectively, signifying that it is an antipathogenic protein.

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References

Leontievsky A, Vares T, Lankinen P, Shergill J, Pozdnyakova N, Myasoedova N . Blue and yellow laccases of ligninolytic fungi. FEMS Microbiol Lett. 1997; 156(1):9-14. DOI: 10.1111/j.1574-6968.1997.tb12698.x. View

Giardina P, Aurilia V, Cannio R, Marzullo L, Amoresano A, Siciliano R . The gene, protein and glycan structures of laccase from Pleurotus ostreatus. Eur J Biochem. 1996; 235(3):508-15. DOI: 10.1111/j.1432-1033.1996.00508.x. View

Eggert C, Temp U, Eriksson K . The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase. Appl Environ Microbiol. 1996; 62(4):1151-8. PMC: 167880. DOI: 10.1128/aem.62.4.1151-1158.1996. View

Sun J, Chen Q, Cao Q, Wu Y, Xu L, Zhu M . A laccase with antiproliferative and HIV-I reverse transcriptase inhibitory activities from the mycorrhizal fungus Agaricus placomyces. J Biomed Biotechnol. 2012; 2012:736472. PMC: 3471028. DOI: 10.1155/2012/736472. View

Baldrian P . Fungal laccases - occurrence and properties. FEMS Microbiol Rev. 2006; 30(2):215-42. DOI: 10.1111/j.1574-4976.2005.00010.x. View

Min K, Kim Y, Kim Y, Jung H, Hah Y . Characterization of a novel laccase produced by the wood-rotting fungus Phellinus ribis. Arch Biochem Biophys. 2001; 392(2):279-86. DOI: 10.1006/abbi.2001.2459. View

Ng T . Peptides and proteins from fungi. Peptides. 2004; 25(6):1055-73. DOI: 10.1016/j.peptides.2004.03.013. View

Wang H, Ng T . Purification of a laccase from fruiting bodies of the mushroom Pleurotus eryngii. Appl Microbiol Biotechnol. 2005; 69(5):521-5. DOI: 10.1007/s00253-005-0086-7. View

Li M, Zhang G, Wang H, Ng T . Purification and characterization of a laccase from the edible wild mushroom Tricholoma mongolicum. J Microbiol Biotechnol. 2010; 20(7):1069-76. DOI: 10.4014/jmb.0912.12033. View

10.

Fang E, Pan W, Wong J, Chan Y, Ye X, Ng T . A new Phaseolus vulgaris lectin induces selective toxicity on human liver carcinoma Hep G2 cells. Arch Toxicol. 2011; 85(12):1551-63. DOI: 10.1007/s00204-011-0698-x. View

11.

Brijwani K, Rigdon A, Vadlani P . Fungal laccases: production, function, and applications in food processing. Enzyme Res. 2010; 2010:149748. PMC: 2962899. DOI: 10.4061/2010/149748. View

12.

Laemmli U, Favre M . Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973; 80(4):575-99. DOI: 10.1016/0022-2836(73)90198-8. View

13.

Fukushima Y, Kirk T . Laccase component of the Ceriporiopsis subvermispora lignin-degrading system. Appl Environ Microbiol. 1995; 61(3):872-6. PMC: 167352. DOI: 10.1128/aem.61.3.872-876.1995. View

14.

Jiang X, Lian M, Han Y, Lv S . Antitumor and immunomodulatory activity of a polysaccharide from fungus Coprinus comatus (Mull.:Fr.) Gray. Int J Biol Macromol. 2013; 58:349-53. DOI: 10.1016/j.ijbiomac.2013.04.033. View

15.

Rouhana-Toubi A, Wasser S, Agbarya A, Fares F . Inhibitory effect of ethyl acetate extract of the shaggy inc cap medicinal mushroom, Coprinus comatus (Higher Basidiomycetes) fruit bodies on cell growth of human ovarian cancer. Int J Med Mushrooms. 2013; 15(5):457-70. DOI: 10.1615/intjmedmushr.v15.i5.40. View

16.

Bourbonnais R, Paice M, Reid I, Lanthier P, Yaguchi M . Lignin oxidation by laccase isozymes from Trametes versicolor and role of the mediator 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) in kraft lignin depolymerization. Appl Environ Microbiol. 1995; 61(5):1876-80. PMC: 167450. DOI: 10.1128/aem.61.5.1876-1880.1995. View

17.

Shin K, Lee Y . Purification and characterization of a new member of the laccase family from the white-rot basidiomycete Coriolus hirsutus. Arch Biochem Biophys. 2001; 384(1):109-15. DOI: 10.1006/abbi.2000.2083. View

18.

Gu C, Zheng F, Long L, Wang J, Ding S . Engineering the expression and characterization of two novel laccase isoenzymes from Coprinus comatus in Pichia pastoris by fusing an additional ten amino acids tag at N-terminus. PLoS One. 2014; 9(4):e93912. PMC: 3977997. DOI: 10.1371/journal.pone.0093912. View

19.

Wang G, Wang J, Fu Y, Bai L, He M, Li B . Systemic treatment with vanadium absorbed by Coprinus comatus promotes femoral fracture healing in streptozotocin-diabetic rats. Biol Trace Elem Res. 2012; 151(3):424-33. DOI: 10.1007/s12011-012-9584-5. View

20.

Hu D, Zhang R, Zhang G, Wang H, Ng T . A laccase with antiproliferative activity against tumor cells from an edible mushroom, white common Agrocybe cylindracea. Phytomedicine. 2010; 18(5):374-9. DOI: 10.1016/j.phymed.2010.07.004. View