Antinociceptive and Anti-inflammatory Activities of Geranium Bellum and Its Isolated Compounds

Overview

Journal BMC Complement Altern Med

Publisher Biomed Central

Specialty Rehabilitation Medicine

Date 2014 Dec 19

PMID 25518981

Citations 11

Authors

Claudia Velazquez-Gonzalez

Raquel Carino-Cortes

Juan A Gayosso de Lucio

Mario I Ortiz

Minarda De la O Arciniega

Diana A Altamirano-Baez

Luis Jimenez- Angeles

Mirandeli Bautista-Avila

Affiliations

Soon will be listed here.

Abstract

Background: Geranium bellum Rose, locally known as "Pata de león", is a perennial plant distributed in the mountains of Hidalgo, Mexico. It is widely used in Mexican traditional medicine to treat fever, pain, and gastrointestinal disorders. To date, there are not published studies regarding the in vivo antinociceptive and anti-inflammatory potential of the acetone-aqueous extract from the aerial parts of G. bellum.

Methods: Antinociceptive effects of the acetone-aqueous G. bellum (AGB) extract and the isolated compounds were assessed using experimental pain models, including thermal nociception like hot plate test, and chemical nociception induced by intraperitoneal acetic acid or subplantar formalin injection in vivo. The anti-inflammatory properties of the extract were studied using systemic administration in carrageenan-induced paw edema.

Results: Intra-gastric administration of AGB (75, 150, and 300 mg/kg) showed a dose-dependent antinociceptive effect in intraperitoneal acetic acid (writhing), thermal nociception in CD1 mice, and subplantar formalin models, as well as anti-inflammatory effect in carrageenan- induced paw edema in Wistar rats. Geraniin and quercetin showed the highest antinociceptive activity in writhing test, whereas ellagic acid was the most active compound in the hot plate model.

Conclusion: These studies provide evidences that G. bellum shows antinociceptive and anti- inflammatory effects, and gives support to its use in treating pain in Mexican traditional medicine.

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References

Ortiz M . Metformin and phenformin block the peripheral antinociception induced by diclofenac and indomethacin on the formalin test. Life Sci. 2011; 90(1-2):8-12. DOI: 10.1016/j.lfs.2011.10.009. View

Moreira J, Klein-Junior L, Filho V, de Campos Buzzi F . Anti-hyperalgesic activity of corilagin, a tannin isolated from Phyllanthus niruri L. (Euphorbiaceae). J Ethnopharmacol. 2013; 146(1):318-23. DOI: 10.1016/j.jep.2012.12.052. View

KASHIWADA Y, Nonaka G, Nishioka I, Chang J, Lee K . Antitumor agents, 129. Tannins and related compounds as selective cytotoxic agents. J Nat Prod. 1992; 55(8):1033-43. DOI: 10.1021/np50086a002. View

Moreira A, Spitzer V, Schapoval E, Schenkel E . Antiinflammatory activity of extracts and fractions from the leaves of Gochnatia polymorpha. Phytother Res. 2000; 14(8):638-40. DOI: 10.1002/1099-1573(200012)14:8<638::aid-ptr681>3.0.co;2-q. View

Ribeiro R, Vale M, Ferreira S, Cunha F . Analgesic effect of thalidomide on inflammatory pain. Eur J Pharmacol. 2000; 391(1-2):97-103. DOI: 10.1016/s0014-2999(99)00918-8. View

Ortiz M, Castaneda-Hernandez G . Examination of the interaction between peripheral lumiracoxib and opioids on the 1% formalin test in rats. Eur J Pain. 2007; 12(2):233-41. DOI: 10.1016/j.ejpain.2007.05.008. View

Dubuisson D, Dennis S . The formalin test: a quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats. Pain. 1977; 4(2):161-174. DOI: 10.1016/0304-3959(77)90130-0. View

. Pain terms: a list with definitions and notes on usage. Recommended by the IASP Subcommittee on Taxonomy. Pain. 1979; 6(3):249. View

Ojewole J . Antinociceptive, anti-inflammatory and antidiabetic properties of Hypoxis hemerocallidea Fisch. & C.A. Mey. (Hypoxidaceae) corm ['African Potato'] aqueous extract in mice and rats. J Ethnopharmacol. 2005; 103(1):126-34. DOI: 10.1016/j.jep.2005.07.012. View

10.

Lu C, Li Y, Li L, Liang L, Shen Y . Anti-inflammatory activities of fractions from Geranium nepalense and related polyphenols. Drug Discov Ther. 2012; 6(4):194-7. View

11.

Seibert K, Zhang Y, Leahy K, Hauser S, Masferrer J, Perkins W . Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. Proc Natl Acad Sci U S A. 1994; 91(25):12013-7. PMC: 45366. DOI: 10.1073/pnas.91.25.12013. View

12.

Shim J, Oh P, Lim K . Anti-inflammatory activity of ethanol extract from Geranium sibiricum Linne. J Ethnopharmacol. 2009; 126(1):90-5. DOI: 10.1016/j.jep.2009.08.004. View

13.

Gayosso-De-Lucio J, Torres-Valencia J, Cerda-Garcia-Rojas C, Joseph-Nathan P . Ellagitannins from Geranium potentillaefolium and G. bellum. Nat Prod Commun. 2010; 5(4):531-4. View

14.

Filho A, Filho V, Olinger L, de Souza M . Quercetin: further investigation of its antinociceptive properties and mechanisms of action. Arch Pharm Res. 2008; 31(6):713-21. DOI: 10.1007/s12272-001-1217-2. View

15.

Couture R, Harrisson M, Vianna R, Cloutier F . Kinin receptors in pain and inflammation. Eur J Pharmacol. 2001; 429(1-3):161-76. DOI: 10.1016/s0014-2999(01)01318-8. View

16.

Malmberg A, Yaksh T . Antinociceptive actions of spinal nonsteroidal anti-inflammatory agents on the formalin test in the rat. J Pharmacol Exp Ther. 1992; 263(1):136-46. View

17.

Carino-Cortes R, Gayosso-De-Lucio J, Ortiz M, Sanchez-Gutierrez M, Garcia-Reyna P, Cilia-Lopez V . Antinociceptive, genotoxic and histopathological study of Heliopsis longipes S.F. Blake in mice. J Ethnopharmacol. 2010; 130(2):216-21. DOI: 10.1016/j.jep.2010.04.037. View

18.

Ponce-Monter H, Fernandez-Martinez E, Ortiz M, Ramirez-Montiel M, Cruz-Elizalde D, Perez-Hernandez N . Spasmolytic and anti-inflammatory effects of Aloysia triphylla and citral, in vitro and in vivo studies. J Smooth Muscle Res. 2011; 46(6):309-19. DOI: 10.1540/jsmr.46.309. View

19.

Mansouri M, Naghizadeh B, Ghorbanzadeh B, Farbood Y . Central and peripheral antinociceptive effects of ellagic acid in different animal models of pain. Eur J Pharmacol. 2013; 707(1-3):46-53. DOI: 10.1016/j.ejphar.2013.03.031. View

20.

Gayosso-De-Lucio J, Torres-Valencia M, Rojo-Dominguez A, Najera-Pena H, Aguirre-Lopez B, Salas-Pacheco J . Selective inactivation of triosephosphate isomerase from Trypanosoma cruzi by brevifolin carboxylate derivatives isolated from Geranium bellum Rose. Bioorg Med Chem Lett. 2009; 19(20):5936-9. DOI: 10.1016/j.bmcl.2009.08.055. View