IL-1β and TNFα Promote Monocyte Viability Through the Induction of GM-CSF Expression by Rheumatoid Arthritis Synovial Fibroblasts
Overview
Pathology
Authors
Affiliations
Background: Macrophages and synovial fibroblasts (SF) are two major cells implicated in the pathogenesis of rheumatoid arthritis (RA). SF could be a source of cytokines and growth factors driving macrophages survival and activation. Here, we studied the effect of SF on monocyte viability and phenotype.
Methods: SF were isolated from synovial tissue of RA patients and CD14+ cells were isolated from peripheral blood of healthy donors. SF conditioned media were collected after 24 hours of culture with or without stimulation with TNFα or IL-1β. Macrophages polarisation was studied by flow cytometry.
Results: Conditioned medium from SF significantly increased monocytes viability by 60% compared to CD14+ cells cultured in medium alone (P < 0.001). This effect was enhanced using conditioned media from IL-1β and TNFα stimulated SF. GM-CSF but not M-CSF nor IL34 blocking antibodies was able to significantly decrease monocyte viability by 30% when added to the conditioned media from IL-1β and TNFα stimulated SF (P < 0.001). Finally, monocyte cultured in presence of SF conditioned media did not exhibit a specific M1 or M2 phenotype.
Conclusion: Overall, rheumatoid arthritis synovial fibroblasts stimulated with proinflammatory cytokines (IL-1β and TNFα) promote monocyte viability via GM-CSF but do not induce a specific macrophage polarization.
Barron A, Fabre T, De S F1000Res. 2024; 13:54.
PMID: 38681509 PMC: 11053351. DOI: 10.12688/f1000research.143472.1.
van Kooten N, Blom A, Teunissen van Manen I, Theeuwes W, Roth J, Gorris M Rheumatology (Oxford). 2024; 64(1):332-343.
PMID: 38216750 PMC: 11701306. DOI: 10.1093/rheumatology/keae020.
Schmidt T, Dahlberg A, Berthold E, Krol P, Arve-Butler S, Ryden E Front Immunol. 2023; 14:1190018.
PMID: 37283752 PMC: 10239926. DOI: 10.3389/fimmu.2023.1190018.
Huang R, Jin M, Liu Y, Lu Y, Zhang M, Yan P Front Immunol. 2023; 14:1098977.
PMID: 36845163 PMC: 9950622. DOI: 10.3389/fimmu.2023.1098977.
Liu K, Ding Y, Wang Y, Zhao Q, Yan L, Xie J Clin Exp Med. 2022; 23(2):397-409.
PMID: 35347503 PMC: 10224837. DOI: 10.1007/s10238-022-00810-7.