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IL-1β and TNFα Promote Monocyte Viability Through the Induction of GM-CSF Expression by Rheumatoid Arthritis Synovial Fibroblasts

Overview
Publisher Wiley
Specialties Biochemistry
Pathology
Date 2014 Dec 9
PMID 25484525
Citations 27
Authors
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Abstract

Background: Macrophages and synovial fibroblasts (SF) are two major cells implicated in the pathogenesis of rheumatoid arthritis (RA). SF could be a source of cytokines and growth factors driving macrophages survival and activation. Here, we studied the effect of SF on monocyte viability and phenotype.

Methods: SF were isolated from synovial tissue of RA patients and CD14+ cells were isolated from peripheral blood of healthy donors. SF conditioned media were collected after 24 hours of culture with or without stimulation with TNFα or IL-1β. Macrophages polarisation was studied by flow cytometry.

Results: Conditioned medium from SF significantly increased monocytes viability by 60% compared to CD14+ cells cultured in medium alone (P < 0.001). This effect was enhanced using conditioned media from IL-1β and TNFα stimulated SF. GM-CSF but not M-CSF nor IL34 blocking antibodies was able to significantly decrease monocyte viability by 30% when added to the conditioned media from IL-1β and TNFα stimulated SF (P < 0.001). Finally, monocyte cultured in presence of SF conditioned media did not exhibit a specific M1 or M2 phenotype.

Conclusion: Overall, rheumatoid arthritis synovial fibroblasts stimulated with proinflammatory cytokines (IL-1β and TNFα) promote monocyte viability via GM-CSF but do not induce a specific macrophage polarization.

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References
1.
Bischof R, Zafiropoulos D, Hamilton J, Campbell I . Exacerbation of acute inflammatory arthritis by the colony-stimulating factors CSF-1 and granulocyte macrophage (GM)-CSF: evidence of macrophage infiltration and local proliferation. Clin Exp Immunol. 2000; 119(2):361-7. PMC: 1905504. DOI: 10.1046/j.1365-2249.2000.01125.x. View

2.
Burmester G, Weinblatt M, McInnes I, Porter D, Barbarash O, Vatutin M . Efficacy and safety of mavrilimumab in subjects with rheumatoid arthritis. Ann Rheum Dis. 2012; 72(9):1445-52. PMC: 3756523. DOI: 10.1136/annrheumdis-2012-202450. View

3.
Yang Y, Hamilton J . Dependence of interleukin-1-induced arthritis on granulocyte-macrophage colony-stimulating factor. Arthritis Rheum. 2001; 44(1):111-9. DOI: 10.1002/1529-0131(200101)44:1<111::AID-ANR15>3.0.CO;2-1. View

4.
Inoue H, Takamori M, Shimoyama Y, Ishibashi H, Yamamoto S, Koshihara Y . Regulation by PGE2 of the production of interleukin-6, macrophage colony stimulating factor, and vascular endothelial growth factor in human synovial fibroblasts. Br J Pharmacol. 2002; 136(2):287-95. PMC: 1573344. DOI: 10.1038/sj.bjp.0704705. View

5.
Stanley E, Cifone M, Heard P, Defendi V . Factors regulating macrophage production and growth: identity of colony-stimulating factor and macrophage growth factor. J Exp Med. 1976; 143(3):631-47. PMC: 2190132. DOI: 10.1084/jem.143.3.631. View