Escherichia Coli Surface Display of Single-chain Antibody VRC01 Against HIV-1 Infection

Overview

Journal Virology

Specialty Microbiology

Date 2014 Dec 9

PMID 25482819

Citations 5

Authors

Lin-Xu Wang

Michael Mellon

Dane Bowder

Meghan Quinn

Danielle Shea

Charles Wood

Shi-Hua Xiang

Affiliations

Soon will be listed here.

Abstract

Human immunodeficiency virus type 1 (HIV-1) transmission and infection occur mainly via the mucosal surfaces. The commensal bacteria residing in these surfaces can potentially be employed as a vehicle for delivering inhibitors to prevent HIV-1 infection. In this study, we have employed a bacteria-based strategy to display a broadly neutralizing antibody VRC01, which could potentially be used to prevent HIV-1 infection. The VRC01 antibody mimics CD4-binding to gp120 and has broadly neutralization activities against HIV-1. We have designed a construct that can express the fusion peptide of the scFv-VRC01 antibody together with the autotransporter β-barrel domain of IgAP gene from Neisseria gonorrhoeae, which enabled surface display of the antibody molecule. Our results indicate that the scFv-VRC01 antibody molecule was displayed on the surface of the bacteria as demonstrated by flow cytometry and immunofluorescence microscopy. The engineered bacteria can capture HIV-1 particles via surface-binding and inhibit HIV-1 infection in cell culture.

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