Enrichment of CD56(dim)KIR + CD57 + Highly Cytotoxic NK Cells in Tumour-infiltrated Lymph Nodes of Melanoma Patients

Overview

Journal Nat Commun

Specialty Biology

Date 2014 Dec 5

PMID 25472612

Citations 78

Authors

Talib Hassan Ali

Simona Pisanti

Elena Ciaglia

Roberta Mortarini

Andrea Anichini

Cinzia Garofalo

Rossana Tallerico

Mario Santinami

Elio Gulletta

Caterina Ietto

Mario Galgani

Giuseppe Matarese

Maurizio Bifulco

Soldano Ferrone

Francesco Colucci

Alessandro Moretta

Klas Karre

Ennio Carbone

Affiliations

Soon will be listed here.

Abstract

An important checkpoint in the progression of melanoma is the metastasis to lymph nodes. Here, to investigate the role of lymph node NK cells in disease progression, we analyze frequency, phenotype and functions of NK cells from tumour-infiltrated (TILN) and tumour-free ipsilateral lymph nodes (TFLN) of the same patients. We show an expansion of CD56(dim)CD57(dim)CD69 + CCR7 +KIR+ NK cells in TILN. TILN NK cells display robust cytotoxic activity against autologous melanoma cells. In the blood of metastatic melanoma patients, the frequency of NK cells expressing the receptors for CXCL8 receptor is increased compared with healthy subjects, and blood NK cells also express the receptors for CCL2 and IL-6. These factors are produced in high amount in TILN and in vitro switch the phenotype of blood NK cells from healthy donors to the phenotype associated with TILN. Our data suggest that the microenvironment of TILN generates and/or recruits a particularly effective NK cell subset.

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