The Muscle Ryanodine Receptor and Its Intrinsic Ca2+ Channel Activity

Overview

Journal J Bioenerg Biomembr

Publisher Springer

Specialties Biochemistry
Biology
Endocrinology

Date 1989 Apr 1

PMID 2546931

Citations 41

Authors

F A Lai

G Meissner

Affiliations

Soon will be listed here.

Abstract

In skeletal and cardiac muscle, contraction is initiated by the rapid release of Ca2+ ions from the intracellular membrane system, sarcoplasmic reticulum. Rapid-mixing vesicle ion flux and planar lipid bilayer-single-channel measurements have shown that Ca2+ release is mediated by a high-conductance, ligand-gated Ca2+ channel. Using the Ca2+ release-specific probe ryanodine, a 30 S protein complex composed of four polypeptides of Mr approximately 400,000 has been isolated. Reconstitution of the purified skeletal and cardiac muscle 30 S complexes into planar lipid bilayers induced single Ca2+ channel currents with conductance and gating kinetics similar to those of native Ca2+ release channels. Electron microscopy revealed structural similarity with the protein bridges ("feet") that span the transverse-tubule-sarcoplasmic reticulum junction. These results suggest that striated muscle contains an intracellular Ca2+ release channel that is identical with the ryanodine receptor and the transverse-tubule-sarcoplasmic reticulum spanning feet structures.

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References

Seiler S, Wegener A, Whang D, Hathaway D, Jones L . High molecular weight proteins in cardiac and skeletal muscle junctional sarcoplasmic reticulum vesicles bind calmodulin, are phosphorylated, and are degraded by Ca2+-activated protease. J Biol Chem. 1984; 259(13):8550-7. View

Fairhurst A, Hasselbach W . Calcium efflux from a heavy sarcotubular fraction. Effects of ryanodine, caffeine and magnesium. Eur J Biochem. 1970; 13(3):504-9. DOI: 10.1111/j.1432-1033.1970.tb00953.x. View

JENDEN D, Fairhurst A . The pharmacology of ryanodine. Pharmacol Rev. 1969; 21(1):1-25. View

Jones L, Besch Jr H, Sutko J, Willerson J . Ryanodine-induced stimulation of net Ca++ uptake by cardiac sarcoplasmic reticulum vesicles. J Pharmacol Exp Ther. 1979; 209(1):48-55. View

Rousseau E, Smith J, Henderson J, Meissner G . Single channel and 45Ca2+ flux measurements of the cardiac sarcoplasmic reticulum calcium channel. Biophys J. 1986; 50(5):1009-14. PMC: 1329827. DOI: 10.1016/S0006-3495(86)83543-3. View

Smith J, Coronado R, Meissner G . Sarcoplasmic reticulum contains adenine nucleotide-activated calcium channels. Nature. 1985; 316(6027):446-9. DOI: 10.1038/316446a0. View

Maclennan D, Holland P . Calcium transport in sarcoplasmic reticulum. Annu Rev Biophys Bioeng. 1975; 4(00):377-404. DOI: 10.1146/annurev.bb.04.060175.002113. View

Somlyo A . The messenger across the gap. Nature. 1985; 316(6026):298-9. DOI: 10.1038/316298b0. View

Pessah I, Waterhouse A, Casida J . The calcium-ryanodine receptor complex of skeletal and cardiac muscle. Biochem Biophys Res Commun. 1985; 128(1):449-56. DOI: 10.1016/0006-291x(85)91699-7. View

10.

Meissner G . Isolation and characterization of two types of sarcoplasmic reticulum vesicles. Biochim Biophys Acta. 1975; 389(1):51-68. DOI: 10.1016/0005-2736(75)90385-5. View

11.

Franzini-Armstrong C . STUDIES OF THE TRIAD : I. Structure of the Junction in Frog Twitch Fibers. J Cell Biol. 2009; 47(2):488-99. PMC: 2108094. DOI: 10.1083/jcb.47.2.488. View

12.

Alderson B, Feher J . The interaction of calcium and ryanodine with cardiac sarcoplasmic reticulum. Biochim Biophys Acta. 1987; 900(2):221-9. DOI: 10.1016/0005-2736(87)90336-1. View

13.

Meissner G, Rousseau E, Lai F . Structural and functional correlation of the trypsin-digested Ca2+ release channel of skeletal muscle sarcoplasmic reticulum. J Biol Chem. 1989; 264(3):1715-22. View

14.

Schneider M . Membrane charge movement and depolarization-contraction coupling. Annu Rev Physiol. 1981; 43:507-17. DOI: 10.1146/annurev.ph.43.030181.002451. View

15.

Saito A, Seiler S, Chu A, Fleischer S . Preparation and morphology of sarcoplasmic reticulum terminal cisternae from rabbit skeletal muscle. J Cell Biol. 1984; 99(3):875-85. PMC: 2113387. DOI: 10.1083/jcb.99.3.875. View

16.

Saito A, Inui M, Radermacher M, Frank J, Fleischer S . Ultrastructure of the calcium release channel of sarcoplasmic reticulum. J Cell Biol. 1988; 107(1):211-9. PMC: 2115172. DOI: 10.1083/jcb.107.1.211. View

17.

Michalak M, Dupraz P, Shoshan-Barmatz V . Ryanodine binding to sarcoplasmic reticulum membrane; comparison between cardiac and skeletal muscle. Biochim Biophys Acta. 1988; 939(3):587-94. DOI: 10.1016/0005-2736(88)90106-x. View

18.

Inui M, Saito A, Fleischer S . Purification of the ryanodine receptor and identity with feet structures of junctional terminal cisternae of sarcoplasmic reticulum from fast skeletal muscle. J Biol Chem. 1987; 262(4):1740-7. View

19.

Fabiato A . Calcium-induced release of calcium from the cardiac sarcoplasmic reticulum. Am J Physiol. 1983; 245(1):C1-14. DOI: 10.1152/ajpcell.1983.245.1.C1. View

20.

Chu A, Sumbilla C, Scales D, Piazza A, Inesi G . Trypsin digestion of junctional sarcoplasmic reticulum vesicles. Biochemistry. 1988; 27(8):2827-33. DOI: 10.1021/bi00408a025. View