Analysis of the H+/sugar Symport in Yeast Under Conditions of Depolarized Plasma Membrane

Overview

Journal J Bioenerg Biomembr

Publisher Springer

Specialties Biochemistry
Biology
Endocrinology

Date 1989 Jun 1

PMID 2545668

Citations 4

Authors

J Severin

P Langel

M Hofer

Affiliations

Soon will be listed here.

Abstract

H+/sugar symport in the obligatory aerobic yeast Rhodotorula glutinis was analyzed under conditions where the plasma membrane was selectively depolarized by the lipophilic cation tetraphenylphosphonium (TPP+). Control experiments showed that this treatment did not impair the transmembrane delta pH, the cell energy charge, and the function of plasma membrane H+-ATPase. The kinetic data were fitted to elementary functions derived from a model constructed on the basis of some simplifying premises for ordered (either C + H+ + S or C + S + H+) and random reaction mechanisms. In addition, the comparison of the kinetic parameters in fully energized and depolarized cells provided information about the free carrier charge. It was concluded that the binding sequence of formation of the ternary carrier/H+/substrate complex follows a random mechanism and that the carrier bears a negative charge.

Citing Articles

Effect of salt stress on sugar uptake in osmotolerant yeasts.

Loray M, de Figueroa L, Hofer M Folia Microbiol (Praha). 1998; 43(2):204-6.

PMID: 9721614 DOI: 10.1007/BF02816515.

Characterization of glucose transport in Schizosaccharomyces pombe.

Heiland S, Lichtenberg-Frate H, Naschen T, Hofer M Folia Microbiol (Praha). 1997; 42(3):225-7.

PMID: 9378412 DOI: 10.1007/BF02818985.

Buffer-stimulated citrate efflux in Penicillium simplicissimum: an alternative charge balancing ion flow in case of reduced proton backflow?.

Burgstaller W, Zanella A, Schinner F Arch Microbiol. 1994; 161(1):75-81.

PMID: 8304821 DOI: 10.1007/BF00248896.

Proton-linked sugar transport systems in bacteria.

Henderson P J Bioenerg Biomembr. 1990; 22(4):525-69.

PMID: 2172229 DOI: 10.1007/BF00762961.

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