Parallel Reaction Monitoring (PRM) and Selected Reaction Monitoring (SRM) Exhibit Comparable Linearity, Dynamic Range and Precision for Targeted Quantitative HDL Proteomics

Overview

Journal J Proteomics

Publisher Elsevier

Specialty Biochemistry

Date 2014 Dec 3

PMID 25449833

Citations 84

Authors

Graziella E Ronsein

Nathalie Pamir

Priska D von Haller

Daniel S Kim

Michael N Oda

Gail P Jarvik

Tomas Vaisar

Jay W Heinecke

Affiliations

Soon will be listed here.

Abstract

Biological Significance: HDL, a complex matrix composed of lipids and proteins, is implicated in cardioprotection. Its cholesterol content correlates inversely with cardiovascular disease and it is the current metric to assess cardiovascular risk. However, the cholesterol content does not capture HDL's complexity and heterogeneity. Devising metrics that better capture HDL's cardioprotective effects, we developed an optimized method for quantification of HDL proteome, using PRM in concert with a single labeled protein as internal standard. The availability of a method that increases sample throughput without compromising the reproducibility, sensitivity, and accuracy could therefore point to better risk assessment for CVD or other diseases.

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