MALAT1 Promotes Colorectal Cancer Cell Proliferation/migration/invasion Via PRKA Kinase Anchor Protein 9

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 2014 Dec 3

PMID 25446987

Citations 116

Authors

Min-Hui Yang

Zhi-Yan Hu

Chuan Xu

Lin-Ying Xie

Xiao-Yan Wang

Shi-You Chen

Zu-Guo Li

Affiliations

Soon will be listed here.

Abstract

Our previous studies have shown that the 3' end of metastasis associated lung adenocarcinoma transcript 1 (MALAT1) is involved in colorectal cancer (CRC) cell proliferation and migration/invasion in vitro. The role and mechanism of MALAT1 in CRC metastasis in vivo, however, remain largely unknown. In the present study, we found that MALAT1 was up-regulated in human primary CRC tissues with lymph node metastasis. Overexpression of MALAT1 via RNA activation promoted CRC cell proliferation, invasion and migration in vitro, and stimulated tumor growth and metastasis in mice in vivo. Conversely, knockdown of MALAT1 inhibited CRC tumor growth and metastasis. MALAT1 regulated at least 243 genes in CRC cells in a genome-wide expression profiling. Among these genes, PRKA kinase anchor protein 9 (AKAP-9) was significantly up-regulated at both mRNA and protein levels. AKAP-9 was highly expressed in CRC cells with metastatic potential and human primary CRC tissues with lymph node metastasis, but not in normal cells or tissues. Importantly, knockdown of AKAP-9 blocked MALAT1-mediated CRC cell proliferation, migration and invasion. These data indicate that MALAT1 may promote CRC tumor development via its target protein AKAP-9.

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