MutSα and MutLα Immunoexpression Analysis in Diagnostic Grading of Oral Epithelial Dysplasia and Squamous Cell Carcinoma

Overview

Journal Oral Surg Oral Med Oral Pathol Oral Radiol

Publisher Elsevier

Specialties Dentistry
General Surgery

Date 2014 Dec 3

PMID 25446502

Citations 8

Authors

M Jessri

A J Dalley

C S Farah

Affiliations

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Abstract

Objectives: This study explored the expression of DNA mismatch repair (MMR) proteins in a range of oral biopsies. We further evaluated the significance of MMR protein expression combined with basic demographic data in differentiating grades of oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC).

Study Design: Immunohistochemical expression of MutSα (hMLH1 and hPMS2) and MutLα (hMSH2 and hMSH6) were compared in 98 formalin-fixed paraffin embedded oral biopsies: 21 normal, 24 mild-dysplasia (MD), 8 moderate-to-severe-dysplasia (SD), and 45 OSCC.

Results: Expression of hMLH1, hPMS2, and hMSH2 was reduced in MD, SD, and OSCC compared with the normal. Reduced hMSH2 immunoreactivity discriminated poorly differentiated OSCC from well-differentiated OSCC. The diagnostic model correctly classified 71.4% of cases and revealed that hPMS2-negative biopsies were more likely to be cancerous (odds ratio [OR], 0.11; 95% confidence interval [CI], 0.000-0.813; P = .040).

Conclusion: The results suggested a diagnostic role for MMR proteins in OED and OSCC.

Citing Articles

Influence of Immunoexpression of Mismatch Repair Complex Proteins on Disease-Free Survival in Non-Surgically Treated Oropharyngeal Squamous Cell Carcinomas.

Coelho L, Dantas T, Barros Silva P, Barbosa J, Teixeira A, Nunes Alves A Head Neck Pathol. 2024; 18(1):125.

PMID: 39601931 PMC: 11602900. DOI: 10.1007/s12105-024-01736-0.

Molecular Biomarkers of Malignant Transformation in Head and Neck Dysplasia.

Ranganath K, Feng A, Franco R, Varvares M, Faquin W, Naunheim M Cancers (Basel). 2022; 14(22).

PMID: 36428690 PMC: 9688631. DOI: 10.3390/cancers14225581.

Molecular landscape of head and neck cancer and implications for therapy.

Farah C Ann Transl Med. 2021; 9(10):915.

PMID: 34164549 PMC: 8184465. DOI: 10.21037/atm-20-6264.

Mismatch Repair Proteins in Oropharyngeal Squamous Cell Carcinoma: A Retrospective Observational Study.

de Oliveira Filho O, Dantas T, Silva-Fernandes I, Cunha M, Nunes Alves A, Mota M Head Neck Pathol. 2021; 15(3):803-816.

PMID: 33501557 PMC: 8384930. DOI: 10.1007/s12105-021-01286-9.

PMS2: a potential prognostic protein marker in oral squamous cell carcinoma.

Decker J, Filho O, Freitas M, Silva-Fernandes I, Dantas T, Campelo C Med Oral Patol Oral Cir Bucal. 2020; 26(4):e451-e458.

PMID: 33247565 PMC: 8254887. DOI: 10.4317/medoral.24303.