The BI4 RNA Mitochondrial Maturase of Saccharomyces Cerevisiae Can Stimulate Intra-chromosomal Recombination in Escherichia Coli

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1989 Mar 1

PMID 2543908

Citations 9

Authors

V Goguel

A Bailone

R Devoret

C Jacq

Affiliations

Soon will be listed here.

Abstract

When the bI4 RNA maturase, encoded by the fourth intron of the mitochondrial cytochrome b gene of Saccharomyces cerevisiae, was expressed in Escherichia coli, formation of intra-chromosomal Lac+ recombinants was stimulated threefold. This "hyper-rec" phenotype was recA as well as recBCD dependent. The most active form of the bI4 maturase stimulated homologous recombination whereas splicing deficient mutants of bI4 maturase were either deficient in or unable to stimulate homologous recombination.

Citing Articles

The biology of yeast mitochondrial introns.

Pel H, Grivell L Mol Biol Rep. 1993; 18(1):1-13.

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A mitochondrial group-I intron in fission yeast encodes a maturase and is mobile in crosses.

Schafer B, Wilde B, Massardo D, Manna F, Del Giudice L, Wolf K Curr Genet. 1994; 25(4):336-41.

PMID: 8082176 DOI: 10.1007/BF00351487.

Mutations in the mitochondrial split gene COXI are preferentially located in exons: a mapping study of 170 mutants.

Netter P, Robineau S, Lemaire C Mol Gen Genet. 1995; 246(4):445-54.

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DNA sequence analysis of the apocytochrome b gene of Podospora anserina: a new family of intronic open reading frame.

Cummings D, Michel F, McNally K Curr Genet. 1989; 16(5-6):407-18.

PMID: 2611913 DOI: 10.1007/BF00340720.

Synthesis and function of the mitochondrial intron--encoded bI4 RNA maturase from Saccharomyces cerevisiae. Effects of upstream frame-shift mutations.

Goguel V, Perea J, Jacq C Curr Genet. 1989; 16(4):241-6.

PMID: 2560681 DOI: 10.1007/BF00422109.

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