Anthraquinonyl Glycoside Facilitates the Standardization of Graphene Electrodes for the Impedance Detection of Lectins

Overview

Journal Chem Cent J

Publisher Biomed Central

Specialty Chemistry

Date 2014 Dec 2

PMID 25435901

Citations 4

Authors

Bi-Wen Zhu

Liang Cai

Xiao-Peng He

Guo-Rong Chen

Yi-Tao Long

Affiliations

Soon will be listed here.

Abstract

Background: Construction of electrochemical impedance sensors by the self-assembly technique has become a promising strategy for the 'label-free' detection of protein-ligand interactions. However, previous impedance sensors are devoid of an inherent electrochemical signal, which limits the standardization of the sensors for protein recognition in a reproducible manner.

Results: We designed and synthesized an anthraquinonyl glycoside (AG) where the anthraquinone (AQ) moiety can bind to the surface of a graphene-based working electrode while the glycoside serving as a ligand for lectin. By measuring the inherent voltammetric signal of AQ, the glycosides decorated on the working electrode could be simply quantified to obtain electrodes with a unified signal window. Subsequently, impedance analysis showed that the 'standardized' electrodes gave a reproducible electrochemical response to a selective lectin with no signal variation in the presence of unselective proteins.

Conclusion: Anthraquinone-modified ligands could be used to facilitate the standardization of electrochemical impedance sensors for the reproducible, selective analysis of ligand-protein interactions.

Citing Articles

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Dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes.

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