Improvement of DNA Transfer Frequency and Transposon Mutagenesis of Erwinia Carotovora Subsp. Betavasculorum

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1989 Apr 1

PMID 2543291

Citations 1

Authors

M Rella

P E Axelrood

A R Weinhold

M N Schroth

Affiliations

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Abstract

The production of antibiotics and their role in microbial competition under natural conditions can be readily studied by the use of transposon mutants. Several antibiotic-producing strains of Erwinia carotovora subsp. betavasculorum were unable to accept foreign DNA. A plasmid delivery system was developed, using ethyl methanesulfonate mutagenesis, which entailed isolating E. carotovora subsp. betavasculorum mutants able to accept foreign DNA and transfer it to other strains. This enabled transposon mutagenesis of a wild-type antibiotic-producing strain of E. carotovora subsp. betavasculorum. Twelve antibiotic-negative mutants were isolated, and one of these showed a reduction in antibiotic production in vitro. Many of these mutants also showed a reduction in their ability to macerate potato tissue. The mutants were classified into four genetic groups on the basis of their genetic and phenotypic characteristics, indicating that several genes are involved in antibiotic biosynthesis by E. carotovora subsp. betavasculorum.

Citing Articles

Derivation of Mutants of Erwinia carotovora subsp. betavasculorum Deficient in Export of Pectolytic Enzymes with Potential for Biological Control of Potato Soft Rot.

Costa J, Loper J Appl Environ Microbiol. 1994; 60(7):2278-85.

PMID: 16349316 PMC: 201644. DOI: 10.1128/aem.60.7.2278-2285.1994.

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