A Spatially and Chemically Defined Platform for the Uniform Growth of Human Pluripotent Stem Cells

Overview

Journal Mater Sci Eng C Mater Biol Appl

Publisher Elsevier

Specialties Biomedical Engineering
Biotechnology

Date 2014 Nov 28

PMID 25428067

Citations 3

Authors

Steven J Jonas

Jackelyn A Alva

Wade Richardson

Sean P Sherman

Zoran Galic

April D Pyle

Bruce Dunn

Affiliations

Soon will be listed here.

Abstract

We report the design of a chemically defined platform engineered for the culture of human pluripotent stem cells (hPSCs) that supports the long-term maintenance of self-renewing hPSC populations in a more uniform manner than standard culture systems. Microcontact printing (μCP) of alkanethiol self-assembled monolayers (SAMs) was used to spatially direct hPSC adherence. This technique not only establishes control over hPSC colony size and shape but also preserves genetic stability and provides unprecedented uniformity in the pluripotency of hPSC populations that is quantitatively assessed in the present study.

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