MycN Promotes TRPM7 Expression and Cell Migration in Neuroblastoma Through a Process That Involves Polyamines

Overview

Journal FEBS Open Bio

Specialty Biology

Date 2014 Nov 27

PMID 25426416

Citations 7

Authors

Ingo Lange

Dana-Lynn T Koomoa

Affiliations

Soon will be listed here.

Abstract

Neuroblastoma is an extra-cranial solid cancer in children. MYCN gene amplification is a prognostic indicator of poor outcome in neuroblastoma. Recent studies have shown that the multiple steps involved in cell migration are dependent on the availability of intracellular calcium (Ca(2+)). Although significant advances have been made in understanding the role of Ca(2+) during migration, little has been achieved towards understanding its impact on the progression of diseases such as cancer. Interestingly, previous studies showed that cancer cell migration is regulated by TRPM7, a calcium-permeable ion channel. The objective of the current study was to elucidate the mechanism by which MycN promotes NB cell migration and the mechanism regulating TRPM7 expression. The results showed that MycN increased TRPM7 expression, induced TRPM7 channel activity, increased intracellular Ca(2+) signaling, and promoted cell migration in NB cells. The results also showed that inhibition or down-regulation of ornithine decarboxylase (ODC) inhibited TRPM7 expression, a process that was reversed by spermidine. Overall, this study provides evidence that MycN promotes TRPM7 expression and cell migration through a mechanism that involves ODC synthesis of polyamines.

Citing Articles

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Koles L, Ribiczey P, Szebeni A, Kadar K, Zelles T, Zsembery A Int J Mol Sci. 2024; 25(2).

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Mechanisms involved in selecting and maintaining neuroblastoma cancer stem cell populations, and perspectives for therapeutic targeting.

Farina A, Cappabianca L, Zelli V, Sebastiano M, Mackay A World J Stem Cells. 2021; 13(7):685-736.

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Activation of cell migration via morphological changes in focal adhesions depends on shear stress in MYCN-amplified neuroblastoma cells.

Hiraiwa T, Yamada T, Miki N, Funahashi A, Hiroi N J R Soc Interface. 2019; 16(152):20180934.

PMID: 30836897 PMC: 6451396. DOI: 10.1098/rsif.2018.0934.

FTY-720 induces apoptosis in neuroblastoma via multiple signaling pathways.

Lange I, Espinoza-Fuenzalida I, Ali M, Serrano L, Koomoa D Oncotarget. 2018; 8(66):109985-109999.

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TRPM7 is overexpressed in bladder cancer and promotes proliferation, migration, invasion and tumor growth.

Gao S, Kong C, Zhang Z, Li Z, Bi J, Liu X Oncol Rep. 2017; 38(4):1967-1976.

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