Cre-ativity in the Liver: Transgenic Approaches to Targeting Hepatic Nonparenchymal Cells

Overview

Journal Hepatology

Specialty Gastroenterology

Date 2014 Nov 22

PMID 25412828

Citations 17

Authors

Stephen N Greenhalgh

Kylie P Conroy

Neil C Henderson

Affiliations

Soon will be listed here.

Abstract

Rapid evolution in transgenic (Tg) mouse technology now permits cell-specific and temporal control of fluorescent cell-labeling and gene inactivation. Here, we discuss the principal strategies that have been utilized to target, label, and manipulate hepatic nonparenchymal cells, with emphasis on the utility of constitutive and inducible Cre-lox systems. We summarize key findings of studies employing Tg technology to target hepatic stellate cells, myofibroblasts, liver sinusoidal endothelial cells, and macrophages to illustrate the power of these approaches in identifying cell-specific molecular mechanisms critical to the pathophysiology of liver disease. Increasing adoption of Tg techniques will help to answer fundamental questions regarding the pathogenesis of hepatic diseases and provide the mechanistic rationale to allow identification of novel drug targets, ultimately translating into effective therapies for patients with liver disease.

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