Biochemical Studies of Soluble Atrial Natriuretic Peptide (ANP) Receptors from Rat Olfactory Bulb and Vascular Smooth Muscle Cells

Overview

Journal Cell Mol Neurobiol

Publisher Springer

Specialties Cell Biology
Molecular Biology
Neurology

Date 1989 Mar 1

PMID 2540912

Authors

C C Glembotski

G M Wildey

T R Gibson

Affiliations

Soon will be listed here.

Abstract

1. Aim. The biochemical characteristics of atrial natriuretic peptide receptors (ANP-R) derived from rat vascular smooth muscle (A-10 cell line) and central nervous system (CNS; olfactory bulb) tissue were compared. 2. Method and Results. ANP-Rs from each source were solubilized with 40 to 65% efficiency utilizing the nonionic detergent Lubrol-PX. Upon solubilization, the ANP-R from each source maintained the ability to bind 125I-ANP (99-126) with a high affinity; Scatchard analysis indicated that the VSMC ANP-R displayed a Kd for the radioligand of approximately 10 pM, whereas the olfactory receptor possessed a Kd of about 165 pM. The Bmax values for the soluble VSMC and olfactory ANP-Rs were 285 and 30 fmol/mg protein, respectively. Competition binding studies indicated that the VSMC ANP-R bound ANP(99-126), ANP(103-126), and ANP(103-123) with similar affinities, whereas the olfactory ANP-R was much more sensitive to changes in the COOH-terminal structure of the competing peptide. The soluble ANP-Rs from VSMC and olfactory were chromatographically indistinguishable on phenyl-, DEAE-, and wheat germ agglutinin-agarose columns. However, the ANP-Rs could be distinguished using GTP-agarose; the olfactory ANP-R was capable of binding to the resin, whereas the VSMC ANP-R was not. 3. Conclusions. Coupled with other studies, these data suggest that the A10 VSMC ANP-R observed in this study may not be coupled to guanylate cyclase and may represent a receptor serving a clearance function, whereas a significant proportion of the olfactory CNS ANP-R appears to be associated with GTP-binding proteins, likely particulate guanylate cyclase, and probably represents a coupled form of the receptor.

Citing Articles

Evidence for a dopaminergic mechanism for the diuretic and natriuretic action of centrally administered atrial natriuretic factor.

Israel A, Torres M, Barbella Y Cell Mol Neurobiol. 1989; 9(3):369-78.

PMID: 2532962 PMC: 11567357. DOI: 10.1007/BF00711416.

References

Glembotski C, Wildey G, Gibson T . Molecular forms of immunoactive atrial natriuretic peptide in the rat hypothalamus and atrium. Biochem Biophys Res Commun. 1985; 129(3):671-8. DOI: 10.1016/0006-291x(85)91944-8. View

Hirata Y, Tomita M, Yoshimi H, Ikeda M . Specific receptors for atrial natriuretic factor (ANF) in cultured vascular smooth muscle cells of rat aorta. Biochem Biophys Res Commun. 1984; 125(2):562-8. DOI: 10.1016/0006-291x(84)90576-x. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Schwartz D, GELLER D, Manning P, Siegel N, Fok K, Smith C . Ser-Leu-Arg-Arg-atriopeptin III: the major circulating form of atrial peptide. Science. 1985; 229(4711):397-400. DOI: 10.1126/science.3160114. View

Gardner D, Vlasuk G, Baxter J, Fiddes J, Lewicki J . Identification of atrial natriuretic factor gene transcripts in the central nervous system of the rat. Proc Natl Acad Sci U S A. 1987; 84(8):2175-9. PMC: 304611. DOI: 10.1073/pnas.84.8.2175. View

Scarborough R, Schenk D, McEnroe G, Arfsten A, Kang L, Schwartz K . Truncated atrial natriuretic peptide analogs. Comparison between receptor binding and stimulation of cyclic GMP accumulation in cultured vascular smooth muscle cells. J Biol Chem. 1986; 261(28):12960-4. View

Kuno T, Andresen J, Kamisaki Y, Waldman S, Chang L, SAHEKI S . Co-purification of an atrial natriuretic factor receptor and particulate guanylate cyclase from rat lung. J Biol Chem. 1986; 261(13):5817-23. View

de Bold A, Borenstein H, Veress A, Sonnenberg H . A rapid and potent natriuretic response to intravenous injection of atrial myocardial extract in rats. Life Sci. 1981; 28(1):89-94. DOI: 10.1016/0024-3205(81)90370-2. View

Quirion R, Dalpe M, De Lean A, Gutkowska J, Cantin M, Genest J . Atrial natriuretic factor (ANF) binding sites in brain and related structures. Peptides. 1984; 5(6):1167-72. DOI: 10.1016/0196-9781(84)90183-9. View

10.

Takayanagi R, Inagami T, Snajdar R, Imada T, Tamura M, Misono K . Two distinct forms of receptors for atrial natriuretic factor in bovine adrenocortical cells. Purification, ligand binding, and peptide mapping. J Biol Chem. 1987; 262(25):12104-13. View

11.

Tanaka I, Misono K, Inagami T . Atrial natriuretic factor in rat hypothalamus, atria and plasma: determination by specific radioimmunoassay. Biochem Biophys Res Commun. 1984; 124(2):663-8. DOI: 10.1016/0006-291x(84)91606-1. View

12.

Leitman D, Andresen J, Kuno T, Kamisaki Y, Chang J, Murad F . Identification of multiple binding sites for atrial natriuretic factor by affinity cross-linking in cultured endothelial cells. J Biol Chem. 1986; 261(25):11650-5. View

13.

GELLER D, Currie M, Wakitani K, Cole B, Adams S, Fok K . Atriopeptins: a family of potent biologically active peptides derived from mammalian atria. Biochem Biophys Res Commun. 1984; 120(2):333-8. DOI: 10.1016/0006-291x(84)91258-0. View

14.

Paul A, Marala R, Jaiswal R, Sharma R . Coexistence of guanylate cyclase and atrial natriuretic factor receptor in a 180-kD protein. Science. 1987; 235(4793):1224-6. DOI: 10.1126/science.2881352. View

15.

Gibson T, Wildey G, Manaker S, Glembotski C . Autoradiographic localization and characterization of atrial natriuretic peptide binding sites in the rat central nervous system and adrenal gland. J Neurosci. 1986; 6(7):2004-11. PMC: 6568602. View

16.

Saavedra J, Correa F, Plunkett L, Israel A, Kurihara M, Shigematsu K . Binding of angiotensin and atrial natriuretic peptide in brain of hypertensive rats. Nature. 1986; 320(6064):758-60. DOI: 10.1038/320758a0. View

17.

Napier M, Arcuri K, Vandlen R . Binding and internalization of atrial natriuretic factor by high-affinity receptors in A10 smooth muscle cells. Arch Biochem Biophys. 1986; 248(2):516-22. DOI: 10.1016/0003-9861(86)90504-7. View

18.

Flynn T, Davies P, Kennedy B, de Bold M, de Bold A . Alignment of rat cardionatrin sequences with the preprocardionatrin sequence from complementary DNA. Science. 1985; 228(4697):323-5. DOI: 10.1126/science.3157217. View

19.

Saper C, Standaert D, Currie M, Schwartz D, GELLER D, NEEDLEMAN P . Atriopeptin-immunoreactive neurons in the brain: presence in cardiovascular regulatory areas. Science. 1985; 227(4690):1047-9. DOI: 10.1126/science.2858127. View

20.

Jacobowitz D, Skofitsch G, KEISER H, Eskay R, Zamir N . Evidence for the existence of atrial natriuretic factor-containing neurons in the rat brain. Neuroendocrinology. 1985; 40(1):92-4. DOI: 10.1159/000124058. View