Microarray Identification of Novel Genes Downstream of Six1, a Critical Factor in Cranial Placode, Somite, and Kidney Development

Overview

Journal Dev Dyn

Publisher Wiley

Specialty Anatomy & Histology

Date 2014 Nov 19

PMID 25403746

Citations 15

Authors

Bo Yan

Karen M Neilson

Ramya Ranganathan

Thomas Maynard

Andrea Streit

Sally A Moody

Affiliations

Soon will be listed here.

Abstract

Background: Six1 plays an important role in the development of several vertebrate organs, including cranial sensory placodes, somites, and kidney. Although Six1 mutations cause one form of branchio-otic syndrome (BOS), the responsible gene in many patients has not been identified; genes that act downstream of Six1 are potential BOS candidates.

Results: We sought to identify novel genes expressed during placode, somite and kidney development by comparing gene expression between control and Six1-expressing ectodermal explants. The expression patterns of 19 of the significantly up-regulated and 11 of the significantly down-regulated genes were assayed from cleavage to larval stages. A total of 28/30 genes are expressed in the otocyst, a structure that is functionally disrupted in BOS, and 26/30 genes are expressed in the nephric mesoderm, a structure that is functionally disrupted in the related branchio-otic-renal (BOR) syndrome. We also identified the chick homologues of five genes and show that they have conserved expression patterns.

Conclusions: Of the 30 genes selected for expression analyses, all are expressed at many of the developmental times and appropriate tissues to be regulated by Six1. Many have the potential to play a role in the disruption of hearing and kidney function seen in BOS/BOR patients.

Citing Articles

Using Xenopus to discover new candidate genes involved in BOR and other congenital hearing loss syndromes.

Neal S, Rajasekaran A, Jusic N, Taylor L, Read M, Alfandari D J Exp Zool B Mol Dev Evol. 2023; 342(3):212-240.

PMID: 37830236 PMC: 11014897. DOI: 10.1002/jez.b.23222.

Time-resolved quantitative proteomic analysis of the developing otic vesicle reveals putative congenital hearing loss candidates.

Baxi A, Nemes P, Moody S iScience. 2023; 26(9):107665.

PMID: 37670778 PMC: 10475516. DOI: 10.1016/j.isci.2023.107665.

The sulfotransferase XB5850668.L is required to apportion embryonic ectodermal domains.

Marchak A, Neilson K, Majumdar H, Yamauchi K, Klein S, Moody S Dev Dyn. 2023; 252(12):1407-1427.

PMID: 37597164 PMC: 10842325. DOI: 10.1002/dvdy.648.

Repressive Interactions Between Transcription Factors Separate Different Embryonic Ectodermal Domains.

Klein S, Tavares A, Peterson M, Sullivan C, Moody S Front Cell Dev Biol. 2022; 10:786052.

PMID: 35198557 PMC: 8859430. DOI: 10.3389/fcell.2022.786052.

Generation of a new six1-null line in Xenopus tropicalis for study of development and congenital disease.

Coppenrath K, Tavares A, Shaidani N, Wlizla M, Moody S, Horb M Genesis. 2021; 59(12):e23453.

PMID: 34664392 PMC: 8950083. DOI: 10.1002/dvg.23453.

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