Transferrin-targeted Magnetic/fluorescence Micelles As a Specific Bi-functional Nanoprobe for Imaging Liver Tumor

Overview

Journal Nanoscale Res Lett

Publisher Springer

Specialty Biotechnology

Date 2014 Nov 18

PMID 25400528

Citations 5

Authors

Hui Qi

Zhengzheng Li

Kai Du

Ketao Mu

Qing Zhou

Shuyan Liang

Wenzhen Zhu

Xiangliang Yang

Yanhong Zhu

Affiliations

Soon will be listed here.

Abstract

In order to delineate the location of the tumor both before and during operation, we developed targeted bi-functional polymeric micelles for magnetic resonance (MR) and fluorescence imaging in liver tumors. Hydrophobic superparamagnetic iron oxide nanoparticles (SPIONs) were loaded into the polymeric micelles through self-assembly of an amphiphilic block copolymer poly(ethylene glycol)-poly(ϵ-caprolactone). After, transferrin (Tf) and near-infrared fluorescence molecule Cy5.5 were conjugated onto the surface of the polymeric micelles to obtain the nanosized probe SPIO@PEG-b-PCL-Tf/Cy5.5 (SPPTC). Imaging capabilities of this nanoprobe were evaluated both in vitro and in vivo. The accumulation of SPPTC in HepG2 cells increased over SPIO@PEG-b-PCL-Cy5.5 (SPPC) by confocal microscopy. The targeted nanoprobe SPPTC possessed favorable properties on the MR and fluorescence imaging both in vitro and in vivo. The MTT results showed that the nanoprobes were well tolerated. SPPTC had the potential for pre-operation evaluation and intra-operation navigation of tumors in clinic.

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