Specific Deficiency of Plzf Paralog, Zbtb20, in Sertoli Cells Does Not Affect Spermatogenesis and Fertility in Mice

Overview

Journal Sci Rep

Specialty Science

Date 2014 Nov 15

PMID 25395169

Citations 10

Authors

Xiaohua Jiang

Huan Zhang

Shi Yin

Yuanwei Zhang

Weimei Yang

Wei Zheng

Liu Wang

Zheng Wang

Ihtisham Bukhari

Howard J Cooke

Furhan Iqbal

Qinghua Shi

Affiliations

Soon will be listed here.

Abstract

Ztbt20 is a POK family transcription factor and primarily functions through its conserved C2H2 Krüppel type zinc finger and BTB/POZ domains. The present study was designed to define the function of the Zbtb20, in vivo, during mouse spermatogenesis. Immunohistochemical studies revealed that ZBTB20 protein was localized specifically in the nuclei of Sertoli cells in seminiferous tubules. To investigate its role during spermatogenesis, we crossed Amh-Cre transgenic mice with Zbtb20 floxp mice to generate conditionally knockout mice (cKO) in which Zbtb20 was specifically deleted in Sertoli cells. The cKO mice were fertile and did not show any detectable abnormalities in spermatogenesis. Taken together, though specific deletion of transcription factor Zbtb20 in Sertoli cells has no apparent influence on spermatogenesis, its specific localization in Sertoli cells makes Zbtb20 a useful marker for the identification of Sertoli cells in seminiferous tubules.

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