Eupatorin-induced Cell Death in Human Leukemia Cells is Dependent on Caspases and Activates the Mitogen-activated Protein Kinase Pathway

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Nov 13

PMID 25390937

Citations 15

Authors

Sara Estevez

Maria Teresa Marrero

Jose Quintana

Francisco Estevez

Affiliations

Soon will be listed here.

Abstract

Eupatorin is a naturally occurring flavone that inhibits cell proliferation in human tumor cells. Here we demonstrate that eupatorin arrests cells at the G2-M phase of the cell cycle and induces apoptotic cell death involving activation of multiple caspases, mitochondrial release of cytochrome c and poly(ADP-ribose) polymerase cleavage in human leukemia cells. This flavonoid induced the phosphorylation of members of the mitogen-activated protein kinases and cell death was attenuated by inhibition of c-jun N-terminal kinases/stress activated protein kinases. Eupatorin-induced cell death is mediated by both the extrinsic and the intrinsic apoptotic pathways and through a mechanism dependent on reactive oxygen species generation.

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