Prostacyclin As a Potent Effector of Adipose-cell Differentiation

Overview

Journal Biochem J

Specialty Biochemistry

Date 1989 Jan 15

PMID 2539085

Citations 23

Authors

R Negrel

D Gaillard

G Ailhaud

Affiliations

Soon will be listed here.

Abstract

The terminal differentiation of Ob1771 pre-adipose cells induced by arachidonic acid in serum-free hormone-supplemented medium containing insulin, transferrin, growth hormone, tri-iodothyronine and fetuin (5F medium) was strongly diminished in the presence of inhibitors of prostaglandin synthesis, namely aspirin or indomethacin. Carbaprostacyclin, a stable analogue of prostacyclin (prostaglandin I2) known to be synthesized by pre-adipocytes and adipocytes, behaved as an efficient activator of cyclic AMP production and was able, when added to 5F medium, to mimic the adipogenic effect of arachidonic acid. Prostaglandins E2, F2 alpha and D2, unable to affect the cyclic AMP production, failed to substitute for carbaprostacyclin. However, prostaglandin F2 alpha, which is another metabolite of arachidonic acid in pre-adipose and adipose cells, able to promote inositol phospholipid breakdown and protein kinase C activation, potentiated the adipogenic effect of carbaprostacyclin. In addition, carbaprostacyclin enhanced both a limited proliferation and terminal differentiation of adipose precursor cells isolated from rodent and human adipose tissues maintained in primary culture. These results demonstrate the critical role of prostacyclin and prostaglandin F2 alpha on adipose conversion in vitro and suggest a paracrine/autocrine role of both prostanoids in the development of adipose tissue in vivo.

Citing Articles

Generation of monoclonal antibody against 6-Keto PGF and development of ELISA for its quantification in culture medium.

Chowdhury M, Kabir N, Ahmed R, Yokota K, Mullins R, Reza H Biochem Biophys Rep. 2024; 39:101748.

PMID: 38939124 PMC: 11208916. DOI: 10.1016/j.bbrep.2024.101748.

Perinatal Polyunsaturated Fatty Acid Status and Obesity Risk.

Demmelmair H, Koletzko B Nutrients. 2021; 13(11).

PMID: 34836138 PMC: 8625539. DOI: 10.3390/nu13113882.

Fighting Fat With Fat: n-3 Polyunsaturated Fatty Acids and Adipose Deposition in Broiler Chickens.

Kim M, Voy B Front Physiol. 2021; 12:755317.

PMID: 34658934 PMC: 8511411. DOI: 10.3389/fphys.2021.755317.

Identification and characterization of adipose surface epitopes.

Onogi Y, Khalil A, Ussar S Biochem J. 2020; 477(13):2509-2541.

PMID: 32648930 PMC: 7360119. DOI: 10.1042/BCJ20190462.

Overexpression of cyclooxygenase-2 in adipocytes reduces fat accumulation in inguinal white adipose tissue and hepatic steatosis in high-fat fed mice.

Danneskiold-Samsoe N, Sonne S, Larsen J, Hansen A, Fjaere E, Isidor M Sci Rep. 2019; 9(1):8979.

PMID: 31222118 PMC: 6586826. DOI: 10.1038/s41598-019-45062-w.

References

Pilgrim C . DNA synthesis and differentiation in developing white adipose tissue. Dev Biol. 1971; 26(1):69-76. DOI: 10.1016/0012-1606(71)90108-4. View

Gaillard D, Negrel R, Lagarde M, Ailhaud G . Requirement and role of arachidonic acid in the differentiation of pre-adipose cells. Biochem J. 1989; 257(2):389-97. PMC: 1135592. DOI: 10.1042/bj2570389. View

Green H, Kehinde O . Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells. Cell. 1976; 7(1):105-13. DOI: 10.1016/0092-8674(76)90260-9. View

Tateson J, Moncada S, Vane J . Effects of prostacyclin (PGX) on cyclic AMP concentrations in human platelets. Prostaglandins. 1977; 13(3):389-97. DOI: 10.1016/0090-6980(77)90019-3. View

Marcus A, Weksler B, Jaffe E . Enzymatic conversion of prostaglandin endoperoxide H2 and arachidonic acid to prostacyclin by cultured human endothelial cells. J Biol Chem. 1978; 253(20):7138-41. View

Negrel R, Grimaldi P, Ailhaud G . Establishment of preadipocyte clonal line from epididymal fat pad of ob/ob mouse that responds to insulin and to lipolytic hormones. Proc Natl Acad Sci U S A. 1978; 75(12):6054-8. PMC: 393116. DOI: 10.1073/pnas.75.12.6054. View

Gorman R, HAMILTON R, Hopkins N . Stimulation of human foreskin fibroblast adenosine 3':5'-cyclic monophosphate levels by prostacyclin (prostaglandin I2). J Biol Chem. 1979; 254(5):1671-6. View

Aiken J, Shebuski R . Comparison in anesthetized dogs of the anti-aggregatory and hemodynamic effects of prostacyclin and a chemically stable prostacyclin analog, 6a-carba-PGI2 (carbacyclin). Prostaglandins. 1980; 19(4):629-43. DOI: 10.1016/s0090-6980(80)80011-6. View

Claesson H . Prostaglandin I2 synthesis and elevation of cyclic AMP levels in 3T3 fibroblasts. Biochim Biophys Acta. 1980; 618(3):399-406. DOI: 10.1016/0005-2760(80)90258-1. View

10.

AXELROD L, Levine L . Prostacyclin production by isolated adipocytes. Diabetes. 1981; 30(2):163-7. DOI: 10.2337/diab.30.2.163. View

11.

Hopkins N, Gorman R . Regulation of 3T3-L1 fibroblast differentiation by prostacyclin (prostaglandin I2). Biochim Biophys Acta. 1981; 663(2):457-66. DOI: 10.1016/0005-2760(81)90174-0. View

12.

Negrel R, Ailhaud G . Metabolism of arachidonic acid and prostaglandin synthesis in the preadipocyte clonal line ob17. Biochem Biophys Res Commun. 1981; 98(3):768-77. DOI: 10.1016/0006-291x(81)91178-5. View

13.

Cook J, Kozak L . Sn-glycerol-3-phosphate dehydrogenase gene expression during mouse adipocyte development in vivo. Dev Biol. 1982; 92(2):440-8. DOI: 10.1016/0012-1606(82)90189-0. View

14.

Djian P, Grimaldi P, Negrel R, Ailhaud G . Adipose conversion of OB17 preadipocytes. Relationships between cell division and fat cell cluster formation. Exp Cell Res. 1982; 142(2):273-81. DOI: 10.1016/0014-4827(82)90368-8. View

15.

Hyman B, Stoll L, Spector A . Prostaglandin production by 3T3-L1 cells in culture. Biochim Biophys Acta. 1982; 713(2):375-85. DOI: 10.1016/0005-2760(82)90256-9. View

16.

Mitchell M, Cleland W, Smith M, Simpson E, Mendelson C . Inhibition of prostaglandin biosynthesis in human adipose tissue by glucocorticosteroids. J Clin Endocrinol Metab. 1983; 57(4):771-6. DOI: 10.1210/jcem-57-4-771. View

17.

Gaillard D, Negrel R, Cermolacce C, Ailhaud G . Growth of preadipocyte cell lines and cell strains from rodents in serum-free hormone-supplemented medium. In Vitro. 1984; 20(2):79-88. DOI: 10.1007/BF02626647. View

18.

Negrel R, Grimaldi P, Forest C, Ailhaud G . Establishment and characterization of fibroblast-like cell lines derived from adipocytes with the capacity to redifferentiate into adipocyte-like cells. Methods Enzymol. 1985; 109:377-85. DOI: 10.1016/0076-6879(85)09103-0. View

19.

AXELROD L, Minnich A, Ryan C . Stimulation of prostacyclin production in isolated rat adipocytes by angiotensin II, vasopressin, and bradykinin: evidence for two separate mechanisms of prostaglandin synthesis. Endocrinology. 1985; 116(6):2548-53. DOI: 10.1210/endo-116-6-2548. View

20.

Gaillard D, Ailhaud G, Negrel R . Fetuin modulates growth and differentiation of Ob17 preadipose cells in serum-free hormone-supplemented medium. Biochim Biophys Acta. 1985; 846(1):185-91. DOI: 10.1016/0167-4889(85)90125-9. View