Human Immunodeficiency Virus Tat-activated Expression of Poliovirus Protein 2A Inhibits MRNA Translation

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1989 Apr 1

PMID 2538833

Citations 24

Authors

X H Sun

D Baltimore

Affiliations

Soon will be listed here.

Abstract

To study the effect of poliovirus protein 2A on cellular RNA translation, the tat control system of human immunodeficiency virus (HIV) was used. Protein 2A was expressed from a plasmid construct (pHIV/2A) incorporating the HIV long terminal repeat. Protein synthesis was measured by using chloramphenicol acetyltransferase as a reporter gene driven by the Rous sarcoma virus long terminal repeat. When HIV/2A was cotransfected with the reporter, addition of a tat-producing plasmid caused at least a 50-fold drop in chloramphenicol acetyltransferase synthesis. A HeLa cell line carrying HIV/2A was established. In it, tat expression caused more than a 10-fold drop in chloramphenicol acetyltransferase synthesis from the reporter plasmid. Furthermore, 2A induction by tat caused cleavage of the cellular translation factor P220, a part of eukaryotic translation initiation factor 4F. Thus protein 2A can, by itself, carry out the inhibition of cellular protein synthesis characteristic of a poliovirus infection. Also, the HIV tat activation provides a very effective method to control gene expression in mammalian cells.

Citing Articles

The multifaceted poliovirus 2A protease: regulation of gene expression by picornavirus proteases.

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Translational control by viral proteinases.

Lloyd R Virus Res. 2005; 119(1):76-88.

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Cleavage of poly(A)-binding protein by poliovirus 3C protease inhibits host cell translation: a novel mechanism for host translation shutoff.

Kuyumcu-Martinez N, Van Eden M, Younan P, Lloyd R Mol Cell Biol. 2004; 24(4):1779-90.

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Efficient cleavage of ribosome-associated poly(A)-binding protein by enterovirus 3C protease.

Kuyumcu-Martinez N, Joachims M, Lloyd R J Virol. 2002; 76(5):2062-74.

PMID: 11836384 PMC: 135927. DOI: 10.1128/jvi.76.5.2062-2074.2002.

A stable HeLa cell line that inducibly expresses poliovirus 2A(pro): effects on cellular and viral gene expression.

Barco A, Feduchi E, Carrasco L J Virol. 2000; 74(5):2383-92.

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