The Effect of Antibiotics on Associated Bacterial Community of Stored Product Mites

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Nov 12

PMID 25387104

Citations 15

Authors

Jan Kopecky

Marta Nesvorna

Marketa Mareckova-Sagova

Jan Hubert

Affiliations

Soon will be listed here.

Abstract

Background: Bacteria are associated with the gut, fat bodies and reproductive organs of stored product mites (Acari: Astigmata). The mites are pests due to the production of allergens. Addition of antibiotics to diets can help to characterize the association between mites and bacteria.

Methodology And Principal Findings: Ampicillin, neomycin and streptomycin were added to the diets of mites and the effects on mite population growth (Acarus siro, Lepidoglyphus destructor and Tyrophagus putrescentiae) and associated bacterial community structure were assessed. Mites were treated by antibiotic supplementation (1 mg g(-1) of diet) for 21 days and numbers of mites and bacterial communities were analyzed and compared to the untreated control. Bacterial quantities, determined by real-time PCR, significantly decreased in antibiotic treated specimens from 5 to 30 times in A. siro and T. putrescentiae, while no decline was observed in L. destructor. Streptomycin treatment eliminated Bartonella-like bacteria in the both A. siro and T. putrescentiae and Cardinium in T. putrescentiae. Solitalea-like bacteria proportion increased in the communities of neomycin and streptomycin treated A. siro specimens. Kocuria proportion increased in the bacterial communities of ampicillin and streptomycin treated A. siro and neomycin and streptomycin treated L. destructor.

Conclusions/significance: The work demonstrated the changes of mite associated bacterial community under antibiotic pressure in pests of medical importance. Pre-treatment of mites by 1 mg g(-1) antibiotic diets improved mite fitness as indicated accelerated population growth of A. siro pretreated streptomycin and neomycin and L. destructor pretreated by neomycin. All tested antibiotics supplemented to diets caused the decrease of mite growth rate in comparison to the control diet.

Citing Articles

A novel -like bacterium forms an interdependent mutualistic symbiosis with its host, the stored-product mite .

Xiong Q, Sopko B, Klimov P, Hubert J mSystems. 2024; 9(3):e0082923.

PMID: 38380907 PMC: 10949449. DOI: 10.1128/msystems.00829-23.

Mixta mediterraneensis as a novel and abundant gut symbiont of the allergen-producing domestic mite Blomia tropicalis.

Erban T, Sopko B, Klimov P, Hubert J Exp Appl Acarol. 2024; 92(2):161-181.

PMID: 38227156 PMC: 10927894. DOI: 10.1007/s10493-023-00875-3.

Pesticide residue exposure provides different responses of the microbiomes of distinct cultures of the stored product pest mite Acarus siro.

Hubert J, Navratilova B, Sopko B, Nesvorna M, Phillips T BMC Microbiol. 2022; 22(1):252.

PMID: 36261789 PMC: 9580201. DOI: 10.1186/s12866-022-02661-4.

The Negative Effects of Feces-Associated Microorganisms on the Fitness of the Stored Product Mite .

Green S, Nesvorna M, Hubert J Front Microbiol. 2022; 13:756286.

PMID: 35359745 PMC: 8961420. DOI: 10.3389/fmicb.2022.756286.

Characteristic and expression of Hsp70 and Hsp90 genes from Tyrophagus putrescentiae and their response to thermal stress.

Wang J, Que S, Liu X, Jin M, Xin T, Zou Z Sci Rep. 2021; 11(1):11672.

PMID: 34083669 PMC: 8175703. DOI: 10.1038/s41598-021-91206-2.

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