Genotoxicity and Cytotoxicity of Sevoflurane in Two Human Cell Lines in Vitro with Ionizing Radiation

Overview

Journal Colomb Med (Cali)

Specialties General Medicine
Public Health

Date 2014 Nov 12

PMID 25386035

Authors

Miguel Alcaraz

Samuel Quesada

David Armero

Rocio Martin-Gil

Amparo Olivares

G Daniel Achel

Affiliations

Soon will be listed here.

Abstract

Objective: To determine the in vitro toxicity of different concentrations of sevoflurane in cells exposed to X-ray.

Methods: The genotoxic effects of sevofluorane were studied by means of the micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes. Subsequently, its cytotoxic effects on PNT2 (normal prostate) cells was determined using the cell viability test (MTT) and compared with those induced by different doses of X-rays.

Results: A dose- and time-dependent cytotoxic effect of sevofluorane on PNT2 cells was determined (p >0.001) and a dose-dependent genotoxic effect of sevofluorane was established (p >0.001). However, at volumes lower than 30 μL of sevofluorane at 100%, a non-toxic effect on PNT2 cells was shown.

Conclusion: Sevofluorane demonstrates a genotoxic capacity as determined in vitro by micronucleus test in cytokinesis-blocked cells of irradiated human lymphocytes.

References

Alcaraz M, Acevedo C, Castillo J, Benavente-Garcia O, Armero D, Vicente V . Liposoluble antioxidants provide an effective radioprotective barrier. Br J Radiol. 2009; 82(979):605-9. DOI: 10.1259/bjr/30930369. View

Wong C, Liu T, Chye S, Lu F, Liu Y, Lin Z . Sevoflurane-induced oxidative stress and cellular injury in human peripheral polymorphonuclear neutrophils. Food Chem Toxicol. 2006; 44(8):1399-407. DOI: 10.1016/j.fct.2006.03.004. View

Fenech M, Morley A . Measurement of micronuclei in lymphocytes. Mutat Res. 1985; 147(1-2):29-36. DOI: 10.1016/0165-1161(85)90015-9. View

Robbiano L, Mereto E, Migliazzi Morando A, Pastore P, Brambilla G . Increased frequency of micronucleated kidney cells in rats exposed to halogenated anaesthetics. Mutat Res. 1998; 413(1):1-6. DOI: 10.1016/s1383-5718(97)00187-3. View

Luleci N, Sakarya M, Topcu I, Luleci E, Erincler T, Solak M . [Effects of sevofluran on cell division and levels of sister chromatid exchange]. Anasthesiol Intensivmed Notfallmed Schmerzther. 2005; 40(4):213-6. DOI: 10.1055/s-2005-861138. View

Karabiyik L, Sardas S, Polat U, Kocabas N, Karakaya A . Comparison of genotoxicity of sevoflurane and isoflurane in human lymphocytes studied in vivo using the comet assay. Mutat Res. 2001; 492(1-2):99-107. DOI: 10.1016/s1383-5718(01)00159-0. View

Michel F, Constantin J . Sevoflurane inside and outside the operating room. Expert Opin Pharmacother. 2009; 10(5):861-73. DOI: 10.1517/14656560902798752. View

Brozovic G, Orsolic N, Rozgaj R, Kasuba V, Knezevic F, Knezevic A . DNA damage and repair after exposure to sevoflurane in vivo, evaluated in Swiss albino mice by the alkaline comet assay and micronucleus test. J Appl Genet. 2010; 51(1):79-86. DOI: 10.1007/BF03195714. View

Migita T, Mukaida K, Kobayashi M, Hamada H, Kawamoto M . The severity of sevoflurane-induced malignant hyperthermia. Acta Anaesthesiol Scand. 2011; 56(3):351-6. DOI: 10.1111/j.1399-6576.2011.02573.x. View

10.

Wiesner G, Hoerauf K, Schroegendorfer K, Sobczynski P, Harth M, Ruediger H . High-level, but not low-level, occupational exposure to inhaled anesthetics is associated with genotoxicity in the micronucleus assay. Anesth Analg. 2001; 92(1):118-22. DOI: 10.1097/00000539-200101000-00023. View

11.

Castillo J, Benavente-Garcia O, Del Bano M, Lorente J, Alcaraz M, Dato M . Radioprotective Effects Against Chromosomal Damage Induced in Human Lymphocytes by gamma-Rays as a Function of Polymerization Grade of Grape Seed Extracts. J Med Food. 2003; 4(2):117-123. DOI: 10.1089/109662001300341770. View

12.

Bienengraeber M, Weihrauch D, Kersten J, Pagel P, Warltier D . Cardioprotection by volatile anesthetics. Vascul Pharmacol. 2005; 42(5-6):243-52. DOI: 10.1016/j.vph.2005.02.005. View

13.

Castillo J, Benavente-Garcia O, Lorente J, Alcaraz M, Redondo A, Ortuno A . Antioxidant activity and radioprotective effects against chromosomal damage induced in vivo by X-rays of flavan-3-ols (Procyanidins) from grape seeds (Vitis vinifera): comparative study versus other phenolic and organic compounds. J Agric Food Chem. 2000; 48(5):1738-45. DOI: 10.1021/jf990665o. View

14.

Goa K, Noble S, Spencer C . Sevoflurane in paediatric anaesthesia: a review. Paediatr Drugs. 2000; 1(2):127-53. DOI: 10.2165/00128072-199901020-00005. View

15.

Navarro Fernandez J, Alcaraz Banos M, Gomez Moraga A, Vicente Ortega V, Canteras Jordana M . [Absence of chromosomic and genotoxic damage from the radiation dose administered in scintigraphic examinations]. Rev Esp Med Nucl. 2004; 23(3):174-82. DOI: 10.1016/s0212-6982(04)72278-8. View

16.

Alcaraz M, Armero D, Martinez-Beneyto Y, Castillo J, Benavente-Garcia O, Fernandez H . Chemical genoprotection: reducing biological damage to as low as reasonably achievable levels. Dentomaxillofac Radiol. 2011; 40(5):310-4. PMC: 3520264. DOI: 10.1259/dmfr/95408354. View

17.

Del Bano M, Castillo J, Benavente-Garcia O, Lorente J, Martin-Gil R, Acevedo C . Radioprotective-antimutagenic effects of rosemary phenolics against chromosomal damage induced in human lymphocytes by gamma-rays. J Agric Food Chem. 2006; 54(6):2064-8. DOI: 10.1021/jf0581574. View

18.

Vral A, Fenech M, Thierens H . The micronucleus assay as a biological dosimeter of in vivo ionising radiation exposure. Mutagenesis. 2010; 26(1):11-7. DOI: 10.1093/mutage/geq078. View

19.

Szyfter K, Szulc R, Mikstacki A, Stachecki I, Rydzanicz M, Jaloszynski P . Genotoxicity of inhalation anaesthetics: DNA lesions generated by sevoflurane in vitro and in vivo. J Appl Genet. 2004; 45(3):369-74. View

20.

Benavente-Garcia O, Castillo J, Lorente J, Alcaraz M . Radioprotective effects in vivo of phenolics extracted from Olea europaea L. leaves against X-ray-induced chromosomal damage: comparative study versus several flavonoids and sulfur-containing compounds. J Med Food. 2002; 5(3):125-35. DOI: 10.1089/10966200260398152. View