Dual Enhancer Activities of the Cyclic-AMP Responsive Element with Cell Type and Promoter Specificity

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1989 Feb 25

PMID 2537960

Citations 7

Authors

S Ishii

Affiliations

Soon will be listed here.

Abstract

The role of the cyclic-AMP (cAMP) responsive element (CRE) in eukaryotic gene transcription was investigated in several cell lines transfected by constructs containing the chloramphenicol acetyltransferase (CAT) gene linked to the three different promoters, simian virus (SV) 40, human c-Ha-ras-1, or chicken beta-actin promoter, with or without CRE. CRE had inducible enhancer activity only when it was linked to the SV40 promoter and in a few cell lines such as PC12. CRE functioned as a constitutive enhancer with the human c-Ha-ras-1 promoter in all cell lines examined. CRE also had constitutive enhancer activity when it was linked to the chicken beta-actin promoter, but this activity was observed only in KB, HeLa, and A431 cells. The different types of enhancer activities of CRE depending on the cell and promoter may be caused by interaction with different trans-acting factors that were demonstrated by gel retardation analyses.

Citing Articles

ATF-1CREB heterodimer is involved in constitutive expression of the housekeeping Na,K-ATPase alpha 1 subunit gene.

Kobayashi M, Kawakami K Nucleic Acids Res. 1995; 23(15):2848-55.

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Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain.

Maekawa T, Sakura H, Sudo T, Yoshimura T, Fujisawa J, Yoshida M EMBO J. 1989; 8(7):2023-8.

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The cellular transcription factor CREB corresponds to activating transcription factor 47 (ATF-47) and forms complexes with a group of polypeptides related to ATF-43.

Hurst H, Masson N, Jones N, Lee K Mol Cell Biol. 1990; 10(12):6192-203.

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Identification and functional characterisation of the cellular activating transcription factor 43 (ATF-43) protein.

Hurst H, Totty N, Jones N Nucleic Acids Res. 1991; 19(17):4601-9.

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Involvement of tyrosine kinase and protein kinase C in platelet-activating-factor-induced c-fos gene expression in A-431 cells.

Tripathi Y, Lim R, Kandala J, Guntaka R, Shukla S Biochem J. 1992; 286 ( Pt 2):527-33.

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