Virulence Diversity Among Bacteremic Aeromonas Isolates: Ex Vivo, Animal, and Clinical Evidences

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Nov 7

PMID 25375798

Citations 24

Authors

Po-Lin Chen

Chi-Jung Wu

Pei-Jane Tsai

Hung-Jen Tang

Yin-Ching Chuang

Nan-Yao Lee

Ching-Chi Lee

Chia-Wen Li

Ming-Chi Li

Chi-Chung Chen

Hung-Wen Tsai

Chun-Chun Ou

Chang-Shi Chen

Wen-Chien Ko

Affiliations

Soon will be listed here.

Abstract

Background: The objective of this study was to compare virulence among different Aeromonas species causing bloodstream infections.

Methodology/principal Findings: Nine of four species of Aeromonas blood isolates, including A. dhakensis, A. hydrophila, A. veronii and A. caviae were randomly selected for analysis. The species was identified by the DNA sequence matching of rpoD. Clinically, the patients with A. dhakensis bacteremia had a higher sepsis-related mortality rate than those with other species (37.5% vs. 0%, P = 0.028). Virulence of different Aeromonas species were tested in C. elegans, mouse fibroblast C2C12 cell line and BALB/c mice models. C. elegans fed with A. dhakensis and A. caviae had the lowest and highest survival rates compared with other species, respectively (all P values <0.0001). A. dhakensis isolates also exhibited more cytotoxicity in C2C12 cell line (all P values <0.0001). Fourteen-day survival rate of mice intramuscularly inoculated with A. dhakensis was lower than that of other species (all P values <0.0001). Hemolytic activity and several virulence factor genes were rarely detected in the A. caviae isolates.

Conclusions/significance: Clinical data, ex vivo experiments, and animal studies suggest there is virulence variation among clinically important Aeromonas species.

Citing Articles

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References

Kelly K, Koehler J, Ashdown L . Spectrum of extraintestinal disease due to Aeromonas species in tropical Queensland, Australia. Clin Infect Dis. 1993; 16(4):574-9. DOI: 10.1093/clind/16.4.574. View

Gold W, Salit I . Aeromonas hydrophila infections of skin and soft tissue: report of 11 cases and review. Clin Infect Dis. 1993; 16(1):69-74. DOI: 10.1093/clinids/16.1.69. View

Wong C, Heuzenroeder M, Flower R . Inactivation of two haemolytic toxin genes in Aeromonas hydrophila attenuates virulence in a suckling mouse model. Microbiology (Reading). 1998; 144 ( Pt 2):291-298. DOI: 10.1099/00221287-144-2-291. View

Chow J, Yu V . Combination antibiotic therapy versus monotherapy for gram-negative bacteraemia: a commentary. Int J Antimicrob Agents. 1999; 11(1):7-12. DOI: 10.1016/s0924-8579(98)00060-0. View

Heuzenroeder M, Wong C, Flower R . Distribution of two hemolytic toxin genes in clinical and environmental isolates of Aeromonas spp.: correlation with virulence in a suckling mouse model. FEMS Microbiol Lett. 1999; 174(1):131-6. DOI: 10.1111/j.1574-6968.1999.tb13559.x. View

Emekdas G, Aslan G, Tezcan S, Serin M, Yildiz C, Ozturhan H . Detection of the frequency, antimicrobial susceptibility, and genotypic discrimination of Aeromonas strains isolated from municipally treated tap water samples by cultivation and AP-PCR. Int J Food Microbiol. 2006; 107(3):310-4. DOI: 10.1016/j.ijfoodmicro.2005.10.012. View

van der Burg B, Bronkhorst M, Pahlplatz P . Aeromonas hydrophila necrotizing fasciitis. A case report. J Bone Joint Surg Am. 2006; 88(6):1357-60. DOI: 10.2106/JBJS.C.00923. View

Chen P, Chang C, Wu C, Ko N, Lee N, Lee H . Extraintestinal focal infections in adults with nontyphoid Salmonella bacteraemia: predisposing factors and clinical outcome. J Intern Med. 2007; 261(1):91-100. DOI: 10.1111/j.1365-2796.2006.01748.x. View

Aguilera-Arreola M, Hernandez-Rodriguez C, Zuniga G, Figueras M, Garduno R, Castro-Escarpulli G . Virulence potential and genetic diversity of Aeromonas caviae, Aeromonas veronii, and Aeromonas hydrophila clinical isolates from Mexico and Spain: a comparative study. Can J Microbiol. 2007; 53(7):877-87. DOI: 10.1139/W07-051. View

10.

Lee C, Chi C, Lee N, Lee H, Chen C, Chen P . Necrotizing fasciitis in patients with liver cirrhosis: predominance of monomicrobial Gram-negative bacillary infections. Diagn Microbiol Infect Dis. 2008; 62(2):219-25. DOI: 10.1016/j.diagmicrobio.2008.05.016. View

11.

Figueras M, Alperi A, Saavedra M, Ko W, Gonzalo N, Navarro M . Clinical relevance of the recently described species Aeromonas aquariorum. J Clin Microbiol. 2009; 47(11):3742-6. PMC: 2772615. DOI: 10.1128/JCM.02216-08. View

12.

Janda J, Abbott S . The genus Aeromonas: taxonomy, pathogenicity, and infection. Clin Microbiol Rev. 2010; 23(1):35-73. PMC: 2806660. DOI: 10.1128/CMR.00039-09. View

13.

Bogaerts A, Temmerman L, Boerjan B, Husson S, Schoofs L, Verleyen P . A differential proteomics study of Caenorhabditis elegans infected with Aeromonas hydrophila. Dev Comp Immunol. 2010; 34(6):690-8. DOI: 10.1016/j.dci.2010.02.003. View

14.

Wang J, Wang C, Yu Chi C, Ho M, Ho C, Lin P . Clinical presentations, prognostic factors, and mortality in patients with Aeromonas sobria complex bacteremia in a teaching hospital: a 5-year experience. J Microbiol Immunol Infect. 2010; 42(6):510-5. View

15.

Parker J, Shaw J . Aeromonas spp. clinical microbiology and disease. J Infect. 2010; 62(2):109-18. DOI: 10.1016/j.jinf.2010.12.003. View

16.

Daly K, Wolf M, Johnson S, Badylak S . A rabbit model of peripheral compartment syndrome with associated rhabdomyolysis and a regenerative medicine approach for treatment. Tissue Eng Part C Methods. 2011; 17(6):631-40. DOI: 10.1089/ten.TEC.2010.0699. View

17.

Martino M, Fasolato L, Montemurro F, Rosteghin M, Manfrin A, Patarnello T . Determination of microbial diversity of Aeromonas strains on the basis of multilocus sequence typing, phenotype, and presence of putative virulence genes. Appl Environ Microbiol. 2011; 77(14):4986-5000. PMC: 3147379. DOI: 10.1128/AEM.00708-11. View

18.

Aravena-Roman M, Harnett G, Riley T, Inglis T, Chang B . Aeromonas aquariorum is widely distributed in clinical and environmental specimens and can be misidentified as Aeromonas hydrophila. J Clin Microbiol. 2011; 49(8):3006-8. PMC: 3147775. DOI: 10.1128/JCM.00472-11. View

19.

Chuang H, Ho Y, Lay C, Wang L, Tsai Y, Tsai C . Different clinical characteristics among Aeromonas hydrophila, Aeromonas veronii biovar sobria and Aeromonas caviae monomicrobial bacteremia. J Korean Med Sci. 2011; 26(11):1415-20. PMC: 3207043. DOI: 10.3346/jkms.2011.26.11.1415. View

20.

Papadakis V, Poniros N, Katsibardi K, Charissiadou A, Anastasopoulos J, Polychronopoulou S . Fulminant Aeromonas hydrophila infection during acute lymphoblastic leukemia treatment. J Microbiol Immunol Infect. 2012; 45(2):154-7. DOI: 10.1016/j.jmii.2011.09.008. View