Identification of Protein Markers in Patients Infected with Plasmodium Knowlesi, Plasmodium Falciparum and Plasmodium Vivax

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2014 Nov 6

PMID 25372941

Citations 4

Authors

Alan Kang-Wai Mu

Ping Chong Bee

Yee Ling Lau

Yeng Chen

Affiliations

Soon will be listed here.

Abstract

Malaria is caused by parasitic protozoans of the genus Plasmodium and is one of the most prevalent infectious diseases in tropical and subtropical regions. For this reason, effective and practical diagnostic methods are urgently needed to control the spread of malaria. The aim of the current study was to identify a panel of new malarial markers, which could be used to diagnose patients infected with various Plasmodium species, including P. knowlesi, P. vivax and P. falciparum. Sera from malaria-infected patients were pooled and compared to control sera obtained from healthy individuals using the isobaric tags for relative and absolute quantitation (iTRAQ) technique. Mass spectrometry was used to identify serum proteins and quantify their relative abundance. We found that the levels of several proteins were increased in pooled serum from infected patients, including cell adhesion molecule-4 and C-reactive protein. In contrast, the serum concentration of haptoglobin was reduced in malaria-infected individuals, which we verified by western blot assay. Therefore, these proteins might represent infectious markers of malaria, which could be used to develop novel diagnostic tools for detecting P. knowlesi, P. vivax and P. falciparum. However, these potential malarial markers will need to be validated in a larger population of infected individuals.

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References

Armah H, Dodoo A, Wiredu E, Stiles J, Adjei A, Gyasi R . High-level cerebellar expression of cytokines and adhesion molecules in fatal, paediatric, cerebral malaria. Ann Trop Med Parasitol. 2005; 99(7):629-47. DOI: 10.1179/136485905X51508. View

Mu A, Lim B, Hashim O, Shuib A . Detection of differential levels of proteins in the urine of patients with endometrial cancer: analysis using two-dimensional gel electrophoresis and o-glycan binding lectin. Int J Mol Sci. 2012; 13(8):9489-9501. PMC: 3431808. DOI: 10.3390/ijms13089489. View

Imrie H, Fowkes F, Michon P, Tavul L, Hume J, Piper K . Haptoglobin levels are associated with haptoglobin genotype and alpha+ -Thalassemia in a malaria-endemic area. Am J Trop Med Hyg. 2006; 74(6):965-71. View

Sohail M, Kaul A, Raziuddin M, Adak T . Decreased glutathione-S-transferase activity: diagnostic and protective role in vivax malaria. Clin Biochem. 2007; 40(5-6):377-82. DOI: 10.1016/j.clinbiochem.2007.01.005. View

Tuteja R . Malaria - an overview. FEBS J. 2007; 274(18):4670-9. DOI: 10.1111/j.1742-4658.2007.05997.x. View

Armah H, Wilson N, Sarfo B, Powell M, Bond V, Anderson W . Cerebrospinal fluid and serum biomarkers of cerebral malaria mortality in Ghanaian children. Malar J. 2007; 6:147. PMC: 2186349. DOI: 10.1186/1475-2875-6-147. View

Sinha S, Qidwai T, Kanchan K, Anand P, Jha G, Pati S . Variations in host genes encoding adhesion molecules and susceptibility to falciparum malaria in India. Malar J. 2008; 7:250. PMC: 2612678. DOI: 10.1186/1475-2875-7-250. View

Daneshvar C, Davis T, Cox-Singh J, Rafaee M, Zakaria S, Divis P . Clinical and laboratory features of human Plasmodium knowlesi infection. Clin Infect Dis. 2009; 49(6):852-60. PMC: 2843824. DOI: 10.1086/605439. View

Conroy A, Lafferty E, Lovegrove F, Krudsood S, Tangpukdee N, Liles W . Whole blood angiopoietin-1 and -2 levels discriminate cerebral and severe (non-cerebral) malaria from uncomplicated malaria. Malar J. 2009; 8:295. PMC: 2806378. DOI: 10.1186/1475-2875-8-295. View

10.

Chattopadhyay R, Velmurugan S, Chakiath C, Andrews Donkor L, Milhous W, Barnwell J . Establishment of an in vitro assay for assessing the effects of drugs on the liver stages of Plasmodium vivax malaria. PLoS One. 2010; 5(12):e14275. PMC: 3000326. DOI: 10.1371/journal.pone.0014275. View

11.

Ray S, Kamath K, Srivastava R, Raghu D, Gollapalli K, Jain R . Serum proteome analysis of vivax malaria: An insight into the disease pathogenesis and host immune response. J Proteomics. 2011; 75(10):3063-80. DOI: 10.1016/j.jprot.2011.10.018. View

12.

Saad A, Mohamed O, Ali A, Bashir A, Ali N, Elbashir M . Acute-phase proteins in pregnant Sudanese women with severe Plasmodium falciparum malaria. Trans R Soc Trop Med Hyg. 2012; 106(9):570-2. DOI: 10.1016/j.trstmh.2012.06.004. View

13.

Ray S, Renu D, Srivastava R, Gollapalli K, Taur S, Jhaveri T . Proteomic investigation of falciparum and vivax malaria for identification of surrogate protein markers. PLoS One. 2012; 7(8):e41751. PMC: 3415403. DOI: 10.1371/journal.pone.0041751. View

14.

Mu A, Lim B, Hashim O, Shuib A . Identification of O-glycosylated proteins that are aberrantly excreted in the urine of patients with early stage ovarian cancer. Int J Mol Sci. 2013; 14(4):7923-31. PMC: 3645724. DOI: 10.3390/ijms14047923. View

15.

Mu A, Chan Y, Kang S, Azman S, Zain R, Chai W . Detection of host-specific immunogenic proteins in the saliva of patients with oral squamous cell carcinoma. J Immunoassay Immunochem. 2013; 35(2):183-93. DOI: 10.1080/15321819.2013.836535. View

16.

Du Clos T . Function of C-reactive protein. Ann Med. 2000; 32(4):274-8. DOI: 10.3109/07853890009011772. View

17.

Imrie H, Ferguson D, Day K . Human serum haptoglobin is toxic to Plasmodium falciparum in vitro. Mol Biochem Parasitol. 2003; 133(1):93-8. DOI: 10.1016/j.molbiopara.2003.07.007. View

18.

Nirmalan N, Sims P, Hyde J . Quantitative proteomics of the human malaria parasite Plasmodium falciparum and its application to studies of development and inhibition. Mol Microbiol. 2004; 52(4):1187-99. DOI: 10.1111/j.1365-2958.2004.04049.x. View

19.

Berendt A, Simmons D, Tansey J, Newbold C, Marsh K . Intercellular adhesion molecule-1 is an endothelial cell adhesion receptor for Plasmodium falciparum. Nature. 1989; 341(6237):57-9. DOI: 10.1038/341057a0. View

20.

Nussler A, Pied S, Pontet M, Miltgen F, Renia L, Gentilini M . Inflammatory status and preerythrocytic stages of malaria: role of the C-reactive protein. Exp Parasitol. 1991; 72(1):1-7. DOI: 10.1016/0014-4894(91)90114-c. View