Sequence Independent Duplex DNA Opening Reaction Catalysed by SV40 Large Tumor Antigen

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1989 Jan 11

PMID 2536153

Citations 14

Authors

M Scheffner

R Wessel

H Stahl

Affiliations

Soon will be listed here.

Abstract

Simian virus 40 (SV40) large tumor antigen (T antigen) is mainly localized in the nucleus where it exhibits two biochemical properties: DNA binding and helicase activity. Both activities are necessary for viral DNA replication and may also enable T antigen to modulate cellular growth. Here we present biochemical and electron microscopic evidence that the helicase activity can start at internal sites of fully double-stranded DNA molecules not containing the SV40 origin or replication. Using T antigen specific monoclonal antibodies, this unwinding reaction can be biochemically divided in an initiation (duplex opening) and a propagation step. The duplex opening reaction (as well as the propagation step) does not depend on a specific DNA sequence or secondary structure. In addition, we have found that T antigen forms an ATP dependent nucleoprotein complex at double-stranded DNA, which may be an essential step for the sequence independent duplex DNA opening reaction.

Citing Articles

ATP-dependent minor groove recognition of TA base pairs is required for template melting by the E1 initiator protein.

Schuck S, Stenlund A J Virol. 2007; 81(7):3293-302.

PMID: 17202221 PMC: 1866042. DOI: 10.1128/JVI.02432-06.

Preformed hexamers of SV40 T antigen are active in RNA and origin-DNA unwinding.

Uhlmann-Schiffler H, Seinsoth S, Stahl H Nucleic Acids Res. 2002; 30(14):3192-201.

PMID: 12136101 PMC: 135737. DOI: 10.1093/nar/gkf416.

The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting.

Chen L, Joo W, Bullock P, Simmons D J Virol. 1997; 71(11):8743-9.

PMID: 9343233 PMC: 192339. DOI: 10.1128/JVI.71.11.8743-8749.1997.

The initiator protein E1 binds to the bovine papillomavirus origin of replication as a trimeric ring-like structure.

Sedman J, Stenlund A EMBO J. 1996; 15(18):5085-92.

PMID: 8890182 PMC: 452248.

p53-catalyzed annealing of complementary single-stranded nucleic acids.

Oberosler P, Hloch P, Ramsperger U, Stahl H EMBO J. 1993; 12(6):2389-96.

PMID: 7685274 PMC: 413469. DOI: 10.1002/j.1460-2075.1993.tb05893.x.

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