β-sitosterol Protects Against Carbon Tetrachloride Hepatotoxicity but Not Gentamicin Nephrotoxicity in Rats Via the Induction of Mitochondrial Glutathione Redox Cycling

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2014 Nov 1

PMID 25361427

Citations 15

Authors

Hoi-Shan Wong

Ji-Hang Chen

Pou-Kuan Leong

Hoi-Yan Leung

Wing-Man Chan

Kam-Ming Ko

Affiliations

Soon will be listed here.

Abstract

Previous findings have demonstrated that β-sitosterol (BSS), an active component of Cistanches Herba, protected against oxidant injury in H9c2 cardiomyocytes and in rat hearts by enhancing mitochondrial glutathione redox cycling, possibly through the intermediacy of mitochondrial reactive oxygen species production. We therefore hypothesized that BSS pretreatment can also confer tissue protection against oxidant injury in other vital organs such as liver and kidney of rats. In this study, the effects of BSS pretreatment on rat models of carbon tetrachloride (CCl4) hepatotoxicity and gentamicin nephrotoxicity were investigated. The findings showed that BSS pretreatment protected against CCl4-induced hepatotoxicity, but not gentamicin nephrotoxicity in rats. The hepatoprotection afforded by BSS was associated with the improvement in mitochondrial glutathione redox status, presumably through the glutathione reductase-mediated enhancement in mitochondrial glutathione redox cycling. The hepatoprotection afforded by BSS was also accompanied by the improved mitochondrial functional ability in rat livers. The inability of BSS to protect against gentamicin nephrotoxicity was likely due to the relatively low bioavailability of BSS in rat kidneys. BSS may serve as potential mitohormetic agent for the prevention of oxidative stress-induced injury in livers.

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