Immunomodulatory Effects of ZYM-201 on LPS-stimulated B Cells

Overview

Journal Immune Netw

Specialty Allergy & Immunology

Date 2014 Nov 1

PMID 25360078

Citations 4

Authors

Ye Eun Lee

Soochan Kim

Woong-Jae Jung

Hyung Soo Lee

Mi-Yeon Kim

Affiliations

Soon will be listed here.

Abstract

ZYM-201 is a methyl ester of triterpenoid glycoside from Sanguisorba officinalis which has been used for treatment of inflammatory and metabolic diseases. In this study, immunomodulatory effects of ZYM-201 on B cells were examined in vitro and in vivo. When splenocytes were activated with lipopolysaccharide (LPS), the major population which had shown an increase in cell numbers was B cells. However, when the B cells were treated with ZYM-201 after LPS activation, their cell numbers and the expression of major costimulatory molecules, CD80 and CD86, were decreased. Furthermore, the effect of LPS, which induces activation of NF-κB, was abolished by ZYM-201: LPS-stimulated B cells showed decrease of phosphorylation after treatment of ZYM-201. The same results were shown in vivo experiments. These results suggest that ZYM-201 may play a role in the modulation of inflammatory responses through inhibiting NF-κB activation and downregulating the expression of costimulatory molecules on B cells.

Citing Articles

Sanguisorbae Radix Suppresses Colorectal Tumor Growth Through PD-1/PD-L1 Blockade and Synergistic Effect With Pembrolizumab in a Humanized PD-L1-Expressing Colorectal Cancer Mouse Model.

Lee E, Kim J, Kim T, Kim Y, Pak M, Jeon C Front Immunol. 2021; 12:737076.

PMID: 34659228 PMC: 8511399. DOI: 10.3389/fimmu.2021.737076.

Syk and IRAK1 Contribute to Immunopharmacological Activities of Anthraquinone-2-carboxlic Acid.

Park J, Son Y, Kim M, Cho J Molecules. 2016; 21(6).

PMID: 27338330 PMC: 6272897. DOI: 10.3390/molecules21060809.

Anti-Inflammatory and Antinociceptive Activities of Anthraquinone-2-Carboxylic Acid.

Park J, Kim S, Kim Y, Yang W, Kim Y, Hong S Mediators Inflamm. 2016; 2016:1903849.

PMID: 27057092 PMC: 4735930. DOI: 10.1155/2016/1903849.

Anti-Diabetic and Hepato-Renal Protective Effects of Ziyuglycoside II Methyl Ester in Type 2 Diabetic Mice.

Son D, Hwang S, Kim M, Park U, Kim B Nutrients. 2015; 7(7):5469-83.

PMID: 26198246 PMC: 4517009. DOI: 10.3390/nu7075232.

References

Goun E, Petrichenko V, Solodnikov S, Suhinina T, Kline M, Cunningham G . Anticancer and antithrombin activity of Russian plants. J Ethnopharmacol. 2002; 81(3):337-42. DOI: 10.1016/s0378-8741(02)00116-2. View

Zhang G, Ghosh S . Toll-like receptor-mediated NF-kappaB activation: a phylogenetically conserved paradigm in innate immunity. J Clin Invest. 2001; 107(1):13-9. PMC: 198554. DOI: 10.1172/JCI11837. View

Tsukahara K, Moriwaki S, Fujimura T, Takema Y . Inhibitory effect of an extract of Sanguisorba officinalis L. on ultraviolet-B-induced photodamage of rat skin. Biol Pharm Bull. 2001; 24(9):998-1003. DOI: 10.1248/bpb.24.998. View

East J . The effect of certain plant preparations on the fertility of laboratory mammals. 4. Sanguisorba officinalis L. J Endocrinol. 1955; 12(4):273-6. DOI: 10.1677/joe.0.0120273. View

Choi J, Kim M, Cha B, Yoo E, Yoon K, Lee J . ZYM-201 sodium succinate ameliorates streptozotocin-induced hyperlipidemic conditions. Planta Med. 2011; 78(1):12-7. DOI: 10.1055/s-0031-1280219. View

Liu X, Cui Y, Yu Q, Yu B . Triterpenoids from Sanguisorba officinalis. Phytochemistry. 2005; 66(14):1671-9. DOI: 10.1016/j.phytochem.2005.05.011. View

Collins A, Brodie D, Gilbert R, Iaboni A, Manso-Sancho R, Walse B . The interaction properties of costimulatory molecules revisited. Immunity. 2002; 17(2):201-10. DOI: 10.1016/s1074-7613(02)00362-x. View

Ban J, Nguyen H, Lee H, Cho S, Ju H, Kim J . Neuroprotective properties of gallic acid from Sanguisorbae radix on amyloid beta protein (25--35)-induced toxicity in cultured rat cortical neurons. Biol Pharm Bull. 2008; 31(1):149-53. DOI: 10.1248/bpb.31.149. View

PIERCE J, Read M, Ding H, Luscinskas F, Collins T . Salicylates inhibit I kappa B-alpha phosphorylation, endothelial-leukocyte adhesion molecule expression, and neutrophil transmigration. J Immunol. 1996; 156(10):3961-9. View

10.

Yamamoto Y, Gaynor R . Therapeutic potential of inhibition of the NF-kappaB pathway in the treatment of inflammation and cancer. J Clin Invest. 2001; 107(2):135-42. PMC: 199180. DOI: 10.1172/JCI11914. View

11.

Ogata H, Su I, Miyake K, Nagai Y, Akashi S, Mecklenbrauker I . The toll-like receptor protein RP105 regulates lipopolysaccharide signaling in B cells. J Exp Med. 2000; 192(1):23-9. PMC: 1887709. DOI: 10.1084/jem.192.1.23. View

12.

Kim Y, Chung C, Kim J, Ko K, Park S, Kim J . Anti-wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient. Biosci Biotechnol Biochem. 2008; 72(2):303-11. DOI: 10.1271/bbb.70268. View

13.

Cho J, Yoo E, Cha B, Park H, Rhee M, Han Y . The inhibitory effect of triterpenoid glycosides originating from Sanguisorba officinalis on tissue factor activity and the production of TNF-alpha. Planta Med. 2006; 72(14):1279-84. DOI: 10.1055/s-2006-947257. View

14.

Choi J, Yu T, Cha B, Rhee M, Yoo E, Kim M . Modulatory effects of ZYM-201 sodium succinate on dietary-induced hyperlipidemic conditions. Pharmazie. 2011; 66(10):791-7. View

15.

Giannoukakis N, Bonham C, Qian S, Chen Z, Peng L, Harnaha J . Prolongation of cardiac allograft survival using dendritic cells treated with NF-kB decoy oligodeoxyribonucleotides. Mol Ther. 2000; 1(5 Pt 1):430-7. DOI: 10.1006/mthe.2000.0060. View

16.

Park K, Koh D, Kim K, Park J, Lim Y . Antiallergic activity of a disaccharide isolated from Sanguisorba officinalis. Phytother Res. 2004; 18(8):658-62. DOI: 10.1002/ptr.1545. View

17.

Yin M, Yamamoto Y, Gaynor R . The anti-inflammatory agents aspirin and salicylate inhibit the activity of I(kappa)B kinase-beta. Nature. 1998; 396(6706):77-80. DOI: 10.1038/23948. View

18.

Buss H, Dorrie A, Schmitz M, Hoffmann E, Resch K, Kracht M . Constitutive and interleukin-1-inducible phosphorylation of p65 NF-{kappa}B at serine 536 is mediated by multiple protein kinases including I{kappa}B kinase (IKK)-{alpha}, IKK{beta}, IKK{epsilon}, TRAF family member-associated (TANK)-binding kinase.... J Biol Chem. 2004; 279(53):55633-43. DOI: 10.1074/jbc.M409825200. View

19.

Wang Z, Loo W, Wang N, Chow L, Wang D, Han F . Effect of Sanguisorba officinalis L on breast cancer growth and angiogenesis. Expert Opin Ther Targets. 2012; 16 Suppl 1:S79-89. DOI: 10.1517/14728222.2011.642371. View

20.

Mimaki Y, Fukushima M, Yokosuka A, Sashida Y, Furuya S, Sakagami H . Triterpene glycosides from the roots of Sanguisorba officinalis. Phytochemistry. 2001; 57(5):773-9. DOI: 10.1016/s0031-9422(01)00083-8. View