Glycosphingolipids of Human Urothelial Cell Lines with Different Grades of Transformation

Overview

Journal Glycoconj J

Publisher Springer

Specialties Biochemistry
Endocrinology

Date 1989 Jan 1

PMID 2535491

Citations 9

Authors

M Ugorski

P Pahlsson

D Dus

B Nilsson

C RADZIKOWSKI

Affiliations

Soon will be listed here.

Abstract

Neutral glycolipids and gangliosides from seven human urothelial cell lines, differing in grades of transformation (TGr), were characterized by fast atom bombardment mass spectrometry, exoglycosidase treatment and an immunostaining procedure. The major neutral glycolipids identified in all cell lines studied included CMH, CDH, CTH, globoside and paragloboside, the gangliosides were GM3, GM2, sialosylparagloboside and GD1a. The following observations were made: 1. GM2 was the major ganglioside in the TGrII cell lines (non-tumorigenic, non-invasive), but a minor component in the TGrIII cell lines (tumorigenic, invasive). 2. All components showed C16:0 and C24:0 as major fatty acids, but in the TGrIII cell lines the fatty acid composition of CMH and some of the gangliosides were more complex showing unsaturated and hydroxy-fatty acids as well.

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Ceramide galactosyltransferase (UGT8) is a molecular marker of breast cancer malignancy and lung metastases.

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References

Nicolson G . Cancer metastasis. Organ colonization and the cell-surface properties of malignant cells. Biochim Biophys Acta. 1982; 695(2):113-76. DOI: 10.1016/0304-419x(82)90020-8. View

Hakomori S . Tumor-associated glycolipid antigens, their metabolism and organization. Chem Phys Lipids. 1986; 42(1-3):209-33. DOI: 10.1016/0009-3084(86)90054-x. View

Symington F, Bernstein I, Hakomori S . Monoclonal antibody specific for lactosylceramide. J Biol Chem. 1984; 259(9):6008-12. View

Kannagi R, Nudelman E, Hakomori S . Possible role of ceramide in defining structure and function of membrane glycolipids. Proc Natl Acad Sci U S A. 1982; 79(11):3470-4. PMC: 346442. DOI: 10.1073/pnas.79.11.3470. View

Uchigata Y, Spitalnik S, Tachiwaki O, Salata K, Notkins A . Pancreatic islet cell surface glycoproteins containing Gal beta 1-4GlcNAc-R identified by a cytotoxic monoclonal autoantibody. J Exp Med. 1987; 165(1):124-39. PMC: 2188266. DOI: 10.1084/jem.165.1.124. View

Iwamori M, Sawada K, Hara Y, Nishio M, Fujisawa T, Imura H . Neutral glycosphingolipids and gangliosides of bovine thyroid. J Biochem. 1982; 91(6):1875-87. View

Natoli Jr E, Livingston P, Pukel C, LLOYD K, Wiegandt H, Szalay J . A murine monoclonal antibody detecting N-acetyl- and N-glycolyl-GM2: characterization of cell surface reactivity. Cancer Res. 1986; 46(8):4116-20. View

Kieler J . Invasiveness of transformed bladder epithelial cells. Cancer Metastasis Rev. 1984; 3(3):265-96. DOI: 10.1007/BF00048389. View

Coon J, Weinstein R, Summers J . Blood group precursor T-antigen expression in human urinary bladder carcinoma. Am J Clin Pathol. 1982; 77(6):692-9. DOI: 10.1093/ajcp/77.6.692. View

10.

Hirabayashi Y, Suzuki T, Suzuki Y, Taki T, Matsumoto M, Higashi H . A new method for purification of anti-glycosphingolipid antibody. Avian anti-hematoside (NeuGc) antibody. J Biochem. 1983; 94(1):327-30. DOI: 10.1093/oxfordjournals.jbchem.a134350. View

11.

Orntoft T, Nielsen M, Wolf H, Olsen S, Clausen H, Hakomori S . Blood group ABO and Lewis antigen expression during neoplastic progression of human urothelium. Immunohistochemical study of type 1 chain structures. Cancer. 1987; 60(11):2641-8. DOI: 10.1002/1097-0142(19871201)60:11<2641::aid-cncr2820601112>3.0.co;2-5. View

12.

Seyama Y, Yamakawa T . Chemical structure of glycolipid of guinea pig red blood cell membrane. J Biochem. 1974; 75(4):837-42. DOI: 10.1093/oxfordjournals.jbchem.a130455. View

13.

Hakomori S . A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964; 55:205-8. View

14.

Tai T, Cahan L, Tsuchida T, Saxton R, Irie R, Morton D . Immunogenicity of melanoma-associated gangliosides in cancer patients. Int J Cancer. 1985; 35(5):607-12. DOI: 10.1002/ijc.2910350507. View

15.

Bounias M . N-(1-naphthyl)ethylenediamine dihydrochloride as a new reagent for nanomole quantification of sugars on thin-layer plates by a mathematical calibration process. Anal Biochem. 1980; 106(2):291-5. DOI: 10.1016/0003-2697(80)90523-0. View

16.

Svennerholm L . Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta. 1957; 24(3):604-11. DOI: 10.1016/0006-3002(57)90254-8. View

17.

Young Jr W, Macdonald E, Nowinski R, HAKOMORI S . Production of monoclonal antibodies specific for two distinct steric portions of the glycolipid ganglio-N-triosylceramide (asialo GM2). J Exp Med. 1979; 150(4):1008-19. PMC: 2185672. DOI: 10.1084/jem.150.4.1008. View

18.

Magnani J, Nilsson B, Brockhaus M, Zopf D, Steplewski Z, KOPROWSKI H . A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II. J Biol Chem. 1982; 257(23):14365-9. View

19.

Vilien M, Christensen B, Wolf H, Rasmussen F, Povlsen C . Comparative studies of normal, 'spontaneously' transformed and malignant human urothelium cells in vitro. Eur J Cancer Clin Oncol. 1983; 19(6):775-89. DOI: 10.1016/0277-5379(83)90010-x. View

20.

Kannagi R, Stroup R, Cochran N, Urdal D, Young Jr W, Hakomori S . Factors affecting expression of glycolipid tumor antigens: influence of ceramide composition and coexisting glycolipid on the antigenicity of gangliotriaosylceramide in murine lymphoma cells. Cancer Res. 1983; 43(10):4997-5005. View