Genome-scale Quantitative Characterization of Bacterial Protein Localization Dynamics Throughout the Cell Cycle

Overview

Journal Mol Microbiol

Specialties Microbiology
Molecular Biology

Date 2014 Oct 30

PMID 25353361

Citations 30

Authors

Nathan J Kuwada

Beth Traxler

Paul A Wiggins

Affiliations

Soon will be listed here.

Abstract

Bacterial cells display both spatial and temporal organization, and this complex structure is known to play a central role in cellular function. Although nearly one-fifth of all proteins in Escherichia coli localize to specific subcellular locations, fundamental questions remain about how cellular-scale structure is encoded at the level of molecular-scale interactions. One significant limitation to our understanding is that the localization behavior of only a small subset of proteins has been characterized in detail. As an essential step toward a global model of protein localization in bacteria, we capture and quantitatively analyze spatial and temporal protein localization patterns throughout the cell cycle for nearly every protein in E. coli that exhibits nondiffuse localization. This genome-scale analysis reveals significant complexity in patterning, notably in the behavior of DNA-binding proteins. Complete cell-cycle imaging also facilitates analysis of protein partitioning to daughter cells at division, revealing a broad and robust assortment of asymmetric partitioning behaviors.

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