Supplementation of Seaweeds Extracts Suppresses Azoxymethane-induced Aberrant DNA Methylation in Colon and Liver of ICR Mice

Overview

Journal J Cancer Prev

Specialty Oncology

Date 2014 Oct 23

PMID 25337591

Citations 2

Authors

So Young Bu

Hoonjeong Kwon

Mi-Kyung Sung

Affiliations

Soon will be listed here.

Abstract

Background: Seamustard and seatangle are commonly consumed seaweeds in Korea and rich sources of non-digestible polysaccharides which possess biological activities. However anti-mutagenic and anti-cancer activities of these seaweeds under physiological condition have not been clarified yet. The objective of this study was to investigate the effect of seaweeds consumption on azoxymethane (AOM) -induced DNA methylation at N(7) and O(6) position of guanine base, an indicator of DNA damage related to cancer initiation.

Methods: THIRTY ICR MICE WERE DIVIDED INTO FIVE GROUPS AND FED ONE OF THE FOLLOWING DIETS FOR TWO WEEKS: control diet, diet containing 10% water-soluble or water-insoluble fraction of seamustard or seatangle. After two weeks of experimental diet AOM was injected at 6 hours before sacrifice and N(7)-methylguanine (N(7)-meG) and O(6)-methylguanine (O(6)-meG) from the colon and liver DNA were quantified using a gas chromatography-mass spectroscopy.

Results: Water-soluble fractions of both seamustard and seatangle significantly reduced AOM-induced production of N(7)-meG guanine in colon and liver. Also water-soluble fractions of these seaweeds suppressed the level of methylation at O(6)-guanine of colon and liver directly responsible for tumorigenesis. While water-insoluble fraction of seamustard suppressed the production of N(7)-meG in liver this seaweed fraction decreased O(6)-meG and the ratio of O(6)/N(7)-meG in liver. Water insoluble fraction of seatangle decreased both O(6)- and N(7)-meG in colon and liver. Supplementation of all seaweeds extracts increased fecal weight of animals and the increase of fecal weight by water-insoluble fraction of seaweeds were higher than that by water-soluble fraction.

Conclusion: Seamustard and seatangle intake may effectively prevent colon and liver carcinogenesis by decreasing DNA damage and the mechanism of inhibiting carcinogenesis by seaweeds in a long term study are warranted.

Citing Articles

Red Seaweed (Hypnea Bryodies and Melanothamnus Somalensis) Extracts Counteracting Azoxymethane-Induced Hepatotoxicity in Rats.

Ibrahim Waly M, Al Alawi A, Al Marhoobi I, Rahman M Asian Pac J Cancer Prev. 2017; 17(12):5071-5074.

PMID: 28122436 PMC: 5454638. DOI: 10.22034/APJCP.2016.17.12.5071.

High-fat diets rich in saturated fat protect against azoxymethane/dextran sulfate sodium-induced colon cancer.

Enos R, Velazquez K, McClellan J, Cranford T, Nagarkatti M, Nagarkatti P Am J Physiol Gastrointest Liver Physiol. 2016; 310(11):G906-19.

PMID: 27033117 PMC: 4935479. DOI: 10.1152/ajpgi.00345.2015.

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